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Fig. S1. Map of the Acheron River catchment illustrating the position of sample sites L1–L8 (on the Little River), S1–S4 (on the Steavenson River), T1–T3 (on the Taggerty River) and A1–A3 on the Acheron River.

Fig. S2. Average daily discharge for the Acheron River at the township of Taggerty during the period of this study.

Fig. S3. Two sets of imaginary data that illustrate an important and common assumption behind the standard calculation of drift densities, in which drift numbers are divided by the volume of water sampled by nets.

Fig. S4. The total number of drifting hydropsychids of two species, Asmicridea sp. AV1 and Smicrophylax sp. AV2, captured during five sampling occasions over a week for 21 site/times plotted against the total volume of water sampled by drift nets during that time.

Fig. S5. A diagram illustrating a cross-section through a channel at the top of sites.

Table S1. Locations and characteristics of the 24 site/time combinations, giving the week of sampling, width of the channel at upstream edge of site, number of drift nets used, average water depth through the cross-section, average water velocity passing through nets over the week, cross-sectional area of the water column at the upstream edge of sites, and the proportion of the cross-sectional area sampled by nets

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