Using quantitative real-time PCR to detect salmonid prey in scats of grey Halichoerus grypus and harbour Phoca vitulina seals in Scotland – an experimental and field study
Article first published online: 7 DEC 2007
© 2007 The Authors. Journal compilation © 2007 British Ecological Society
Journal of Applied Ecology
Volume 45, Issue 2, pages 632–640, April 2008
How to Cite
Matejusová, I., Doig, F., Middlemas, S. J., Mackay, S., Douglas, A., Armstrong, J. D., Cunningham, C. O. and Snow, M. (2008), Using quantitative real-time PCR to detect salmonid prey in scats of grey Halichoerus grypus and harbour Phoca vitulina seals in Scotland – an experimental and field study. Journal of Applied Ecology, 45: 632–640. doi: 10.1111/j.1365-2664.2007.01429.x
- Issue published online: 7 DEC 2007
- Article first published online: 7 DEC 2007
- Received 15 November 2006; accepted 14 September 2007Handling Editor: Paul Thompson
- Atlantic salmon;
- Moray Firth;
- prey detection;
- sea trout;
- 1There is considerable debate over the impact of seal predation on salmonid populations in both the Atlantic and Pacific oceans. Conventional hard-part analysis of scats has suggested that salmonids represent a minor component of the diet of grey seals (Halichoerus grypus) and harbour seals (Phoca vitulina) in the UK. However, it is unclear whether this is an accurate reflection of the diet or due to methodological problems. To investigate this issue, we applied quantitative PCR (qPCR) to examine the presence of salmonids in the diet of seals in the Moray Firth, UK, during the summers of 2003 and 2005.
- 2Two qPCR assays were designed to detect Atlantic salmon Salmo salar and sea trout Salmo trutta DNA in field samples and experimentally spiked scats. The proportion of scats sampled in the field that were positive for salmonid DNA was low (ª10%). However, the DNA technique consistently resulted in more positive scats than when hard-part analysis was used.
- 3An experimental study using spiked scat material revealed a highly significant negative relationship between Ct values obtained from the Atlantic salmon qPCR assay and the proportion of Atlantic salmon material added to scats. The Ct value denotes the cycle number at which the increasing fluorescence signal of target DNA crosses a threshold value. Ct values from field-collected seal scats suggested they contained a very low concentration of salmonid remains (1–5%) based on an approximate calibration curve constructed from the experimental data.
- 4Synthesis and applications. The qPCR assay approach was shown to be highly efficient and consistent in detection of salmonids from seal scats, and to be more sensitive than conventional hard-parts analysis. Nevertheless, our results confirm previous studies indicating that salmonids are not common prey for seals in these Scottish estuaries. These studies support current management practice, which focuses on control of the small number of seals that move into key salmonid rivers, rather than targeting the larger groups of animals that haul-out in nearby estuaries.