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Fig. S1. Road impacts on wildlife populations (modified from Jaeger & Fahrig 2004).

Fig. S2. Stages of highway construction in 2006, 2007 and 2008.

Fig. S3. Model fit (estimated vs. observed fish density) for the best overall linear mixed model (Table 1: model 12).

Table S1. Summary statistics for fish body length and environmental characteristics of study reaches upstream and downstream of highway crossings.

Appendix S1. Specification of the Markovian random walk model.

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