*Department of Botany, Ohio University, Athens, OH 45701, USA.
Culture medium for Xanthomonas campestris pv. oryzae
Article first published online: 11 MAR 2008
Journal of Applied Bacteriology
Volume 69, Issue 6, pages 798–805, December 1990
How to Cite
Yuan, W. (1990), Culture medium for Xanthomonas campestris pv. oryzae. Journal of Applied Bacteriology, 69: 798–805. doi: 10.1111/j.1365-2672.1990.tb01576.x
- Issue published online: 11 MAR 2008
- Article first published online: 11 MAR 2008
- Accepted 27 April 1990
Yuan, W. 1990. Culture medium for Xanthomonas campestris pv. oryzae. Journal of Applied Bacteriology69, 798–805.
Studies on nutrient requirements of four Chinese strains of Xanthomonas campestris pv. oryzae in a modified Watanabe's medium led to the development of a new synthetic medium containing sucrose, sodium glutamate, methionine, KH2PO4, NH4C1 and iron chelated with EDTA. The concentration of each ingredient was optimized based on the number of colonies and time required for their appearance. Various concentrations of some nutrients were compared based upon their effects on growth of the pathogen strains and 34 contaminants from rice materials. Tryp-tone enhanced the growth of X. c. oryzae more than that of many contaminants, including Erwinia herbicola. Peptone stimulated growth of X. c. oryzae without promoting excessive contamination. When compared with other media used for X. c. oryzae, the new culture medium enriched with tryptone and peptone gave the highest recovery and earliest appearance of colonies of Chinese strains of this bacterium.