Factorial analysis of tricarboxylic acid cycle intermediates for optimization of zeaxanthin production from Flavobacterium multivorum
Article first published online: 27 JAN 2004
Journal of Applied Microbiology
Volume 96, Issue 3, pages 623–629, March 2004
How to Cite
Bhosale, P., Larson, A.J. and Bernstein, P.S. (2004), Factorial analysis of tricarboxylic acid cycle intermediates for optimization of zeaxanthin production from Flavobacterium multivorum. Journal of Applied Microbiology, 96: 623–629. doi: 10.1111/j.1365-2672.2004.02197.x
- Issue published online: 27 JAN 2004
- Article first published online: 27 JAN 2004
- 2003/363 received 01 May 2003, revised 06 August 2003 and accepted 15 December 2003
- factorial design;
- Flavobacterium multivorum;
- TCA cycle intermediates;
Aims: To study the effect of intermediates of the tricarboxylic acid (TCA) cycle on the production of zeaxanthin from Flavobacterium multivorum in order to optimize production of this xanthophyll carotenoid.
Methods and Results: The concentration of selected TCA cycle intermediates (malic acid, isocitric acid and α-ketoglutarate) was optimized in shake flask culture, using a statistical two-level, three-variable factorial approach. The carotenoid production profile was also studied in the optimized medium at various growth phases. Optimized medium resulted in a sixfold increase in volumetric production of zeaxanthin (10·65 ± 0·63 μg ml−1) using malic acid (6·02 mm), isocitric acid (6·20 mm) and α-ketoglutarate (0·02 mm). The majority of zeaxanthin was produced in the late logarithmic growth phase whereas a substantial amount of β-cryptoxanthin and β-carotene were observed in the early logarithmic phase.
Significance and Impact of the Study: This study demonstrates improvement of zeaxanthin production from F. multivorum which might aid in the commercialization of zeaxanthin production from this microbe.