Identification and prevalence of an enterotoxin-related gene, se-int, in Staphylococcus intermedius isolates from dogs and pigeons
Article first published online: 22 MAR 2004
Journal of Applied Microbiology
Volume 96, Issue 6, pages 1361–1366, June 2004
How to Cite
Futagawa-Saito, K., Suzuki, M., Ohsawa, M., Ohshima, S., Sakurai, N., Ba-Thein, W. and Fukuyasu, T. (2004), Identification and prevalence of an enterotoxin-related gene, se-int, in Staphylococcus intermedius isolates from dogs and pigeons. Journal of Applied Microbiology, 96: 1361–1366. doi: 10.1111/j.1365-2672.2004.02264.x
- Issue published online: 22 MAR 2004
- Article first published online: 22 MAR 2004
- 2003/0825: received 17 September 2003, revised 17 February 2004 and accepted 17 February 2004
- Staphylococcus intermedius
Aims: To determine the prevalence of enterotoxin-producing Staphylococcus intermedius in dogs and pigeons.
Methods and Results: A total of 106 S. intermedius isolates from 44 dogs and 62 pigeons were tested for the production of enterotoxins A, B, C and D by reverse passive latex agglutination (RPLA) and for sec-canine by PCR. Only one isolate from dog was positive for SEC and sec-canine. Screening of sec-canine-negative strains by nested PCR led to the identification of a novel enterotoxin-related gene, se-int. SE-int showed a significant homology (59–61% identity) with SEC and (56·6% identity) SEB. All 44 isolates from dogs and five isolates (8·1%) from pigeons were se-int positive.
Conclusions: While S. intermedius was isolated more frequently from pigeons than from dogs, se-int was more prevalent among the S. intermedius isolates from dogs, compared with the pigeon isolates.
Significance and Impact of the Study: Further characterization of the se-int-positive S. intermedius strains should clarify their pathogenic potential including enterotoxigenicity and zoonotic transmissibility to human beings.