- Top of page
- Materials and methods
- Animals and bacterial strains
- Enterotoxin assay
- PCR and nested PCR
- DNA sequencing and analysis
- Transcription analysis by RT-PCR
- Dot blot analysis
- Prevalence of SEs in staphylococci and S. intermedius isolates from dogs and pigeons
- Identification of a novel enterotoxin-related gene
- Transcriptional analysis
- Screening of se-int and sec-canine by dot blot hybridaization
Aims: To determine the prevalence of enterotoxin-producing Staphylococcus intermedius in dogs and pigeons.
Methods and Results: A total of 106 S. intermedius isolates from 44 dogs and 62 pigeons were tested for the production of enterotoxins A, B, C and D by reverse passive latex agglutination (RPLA) and for sec-canine by PCR. Only one isolate from dog was positive for SEC and sec-canine. Screening of sec-canine-negative strains by nested PCR led to the identification of a novel enterotoxin-related gene, se-int. SE-int showed a significant homology (59–61% identity) with SEC and (56·6% identity) SEB. All 44 isolates from dogs and five isolates (8·1%) from pigeons were se-int positive.
Conclusions: While S. intermedius was isolated more frequently from pigeons than from dogs, se-int was more prevalent among the S. intermedius isolates from dogs, compared with the pigeon isolates.
Significance and Impact of the Study: Further characterization of the se-int-positive S. intermedius strains should clarify their pathogenic potential including enterotoxigenicity and zoonotic transmissibility to human beings.