• Bolton, P.H. and Kearns, D.R. (1978) Spectroscopic properties of ethidium monoazide: a fluorescent photoaffinity label for nucleic acid. Nucleic Acid Res 5, 48914903.
  • Clark, G. (1973) Staining Procedures Used by the Biological Stain Commission, 3rd edn. pp. 264265. Baltimore, MD: The Williams & Wilkins Co.
  • Donnellan, J.E., Nags, E.H. and Levinson, H.S. (1964) Chemically defined, synthetic media for sporulation and for germination and growth of Bacillus subtilis. J Bacteriol 87, 332336.
  • Fischer, G. (1982) Cultivation of mouse cerebellar cells in serum free, hormonally defined media: survival of neurons. Neurosci Lett 28, 325329.
  • Frankfurt, O.S. (1983) Assessment of cell viability by flow cytometric analysis using DNase exclusion. Exp Cell Res 144, 478482.
  • Green, F.J. (1990) The Sigma-Aldrich Handbook of Stains, Dyes and Indicators. Milwaukee, WI: Aldrich Chemical Company.
  • Herman, L. (1997) Detection of viable and dead Listeria monocytogenes by PCR. Food Microbiol 14, 103110.
  • Kwee, W.S., Dommett, T.W., Giles, J.E., Roberts, R. and Smith, R.A.D. (1986) Microbiological parameters during powdered milk manufacture. 1. Variation between processes and stages. Aust J Dairy Technol March, 36.
  • Lewis, J.C. (1972) Germination of bacterial spores by calcium chelates of dipicolinic acid analogues. J Biol Chem 247, 18611868.
  • McGuiggan, J.T.M., McCleery, D.R., Hannan, A. and Gilmour, A. (2002) Aerobic spore-forming bacteria in bulk raw milk: factors influencing the numbers of psychrotrophic, mesophilic and thermophilic Bacillus spores. Int J Dairy Technol 55, 100107.
  • Murphy, P., Lynch, D. and Kelly, P. (1999) Growth of thermophilic spore forming bacilli in milk during the manufacture of low heat powders. Int J Dairy Technol 52, 4550.
  • Nogva, H.K., Dromtorp, S.M., Nissen, H. and Rudi, K. (2003) Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5′-nuclease PCR. Biotechniques 34, 804813.
  • Norton, D.M. and Batt, C.A. (1999) Detection of viable Listeria monocytogenes with a 5′ nuclease PCR assay. Appl Environ Microbiol 65, 21222127.
  • Ronimus, R.S., Parker, L.E., Turner, N., Poudel, S., Rückert, A. and Morgan, H.W. (2003) A RAPD-based comparison of thermophilic bacilli from milk powders. Int J Food Microbiol 85, 4561.
  • Rudi, K., Moen, B., Drømtorp, S.M. and Holck, A.L. (2005) Use of ethidium monoazide and PCR in combination for quantification of viable and dead cells in complex samples. Appl Environ Microbiol 71, 10181024.
  • Rueckert, A., Ronimus, R.S. and Morgan, H.W. (2004) A RAPD-based survey of thermophilic bacilli in milk powders from different countries. Int J Food Microbiol 96, 263272.
  • Rueckert, A., Ronimus, R.S. and Morgan, H.W. (2005) Development of a rapid detection and enumeration method for thermophilic bacilli in milk powders. J Microbiol Methods 60, 155167.
  • Stadhouders, J., Hup, G. and Hassing, F. (1982) The conceptions index and indicator organisms discussed on the basis of the bacteriology of spray-dried milk powder. Neth Milk Dairy J 36, 231260.
  • Thompson, S.S., Harmon, L.G. and Stine, C.M. (1978) Survival of selected organisms during spray drying of skim milk and storage of non-fat dry milk. J Food Prot 41, 1619.
  • Thrane, C., Olsson, S., Wolstrup, J. and Sorensen J. (2000) Direct microscopy of Bacillus endospore germination in soil microcosms. J Appl Microbiol 89, 595598.
  • Waring, M.J. (1965) Complex formation between ethidium bromide and nucleic acids. J Mol Biol 13, 269282.