Present address B. Lafay, UMR CNRS-IRD 2724, Centre IRD, 911 avenue Agropolis, BP 64501, 34394 Montpellier cedex 5, France.
Molecular diversity of rhizobia nodulating the invasive legume Cytisus scoparius in Australia
Article first published online: 25 APR 2006
Journal of Applied Microbiology
Volume 100, Issue 6, pages 1228–1238, June 2006
How to Cite
Lafay, B. and Burdon, J.J. (2006), Molecular diversity of rhizobia nodulating the invasive legume Cytisus scoparius in Australia. Journal of Applied Microbiology, 100: 1228–1238. doi: 10.1111/j.1365-2672.2006.02902.x
- Issue published online: 25 APR 2006
- Article first published online: 25 APR 2006
- 2005/1036: received 12 September 2005, revised 25 October 2005 and accepted 18 November 2005
- Cytisus scoparius;
- plant invasion;
- SSU rDNA
Aims: To contribute to the understanding of Cytisus scoparius success at invading and establishing itself in Australia.
Methods and Results: Root-nodule bacteria isolated from C. scoparius, growing on five different sites and originally introduced to Australia, were compared with isolates from indigenous plants growing in France and isolates from native legumes growing on the same Australian sites as C. scoparius. Small-subunit rDNA from 251 isolates were analysed by PCR-RFLP and representatives from different genospecies were selected for sequencing. Phylogenetic analyses revealed a great diversity of lineages belonging to Bradyrhizobium, with one genospecies being specific for Cytisus both in Australia and in France, Rhizobium and Mesorhizobium and one falling outside the described genera of legume-nodulating bacteria. Principal component analysis showed that the Cytisus Australian rhizobial communities are more similar to each other than to their co-occurring native partners.
Conclusions: Early established rhizobial symbionts may have an increased probability to contribute inoculum for the development of further nodules.
Significance and Impact of the Study: This is a first report comparing rhizobia nodulating C. scoparius in its native and exotic environments. Cytisus scoparius symbionts were identified outside the Bradyrhizobium genus and a new lineage of legume-nodulating bacteria was identified.