Characterization of extracellular polymers synthesized by tropical intertidal biofilm bacteria
Article first published online: 1 AUG 2006
Journal of Applied Microbiology
Volume 102, Issue 1, pages 254–264, January 2007
How to Cite
Ortega-Morales, B.O., Santiago-García, J.L., Chan-Bacab, M.J., Moppert, X., Miranda-Tello, E., Fardeau, M.L., Carrero, J.C., Bartolo-Pérez, P., Valadéz-González, A. and Guezennec, J. (2007), Characterization of extracellular polymers synthesized by tropical intertidal biofilm bacteria. Journal of Applied Microbiology, 102: 254–264. doi: 10.1111/j.1365-2672.2006.03085.x
- Issue published online: 1 AUG 2006
- Article first published online: 1 AUG 2006
- 2005/1297: received 31 October 2005, revised 23 March 2006 and accepted 30 March 2006
- Bacillus sp.;
- Microbacterium sp.;
- tropical intertidal biofilm bacteria
Aim: This study was performed to determine the potential of tropical intertidal biofilm bacteria as a source of novel exopolymers (EPS).
Methods and Results: A screening procedure was implemented to detect EPS-producing biofilm bacteria. Isolates MC3B-10 and MC6B-22, identified respectively as a Microbacterium species and Bacillus species by 16S rDNA and cellular fatty acids analyses, produced different EPS, as evidenced by colorimetric and gas chromatographic analyses. The polymer produced by isolate MC3B-10 displays significant surfactant activity, and may chelate calcium as evidenced by spectroscopic analysis.
Conclusions: Polymer MC3B-10 appears to be a glycoprotein, while EPS MC6B-22 seems to be a true polysaccharide dominated by neutral sugars but with significant concentrations of uronic acids and hexosamines. EPS MC3B-10 possesses a higher surfactant activity than that of commercial surfactants, and given its anionic nature, may chelate cations thus proving useful in bioremediation. The chemical composition of polymer MC6B-22 suggests its potential biomedical application in tissue regeneration.
Significance and Impact of the Study: This is the first report of a Microbacterium species producing EPS with surfactant properties, which expands our knowledge of the micro-organisms capable of producing these biomolecules. Furthermore, this work shows that tropical intertidal environments are a nonpreviously recognized habitat for bioprospecting EPS-producing bacteria, and that these molecules might be involved in ecological roles protecting the cells against dessication.