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Cloning, sequencing and characterization of a urease gene operon from urease-positive thermophilic Campylobacter (UPTC)

  1. Y. Kakinuma1,
  2. H. Iida1,
  3. T. Sekizuka1,
  4. K. Usui1,
  5. O. Murayama1,
  6. S. Takamiya2,
  7. B.C. Millar3,
  8. J.E. Moore3,
  9. M. Matsuda1

Article first published online: 9 NOV 2006

DOI: 10.1111/j.1365-2672.2006.03212.x

Issue

Journal of Applied Microbiology

Journal of Applied Microbiology

Volume 103, Issue 1, pages 252–260, July 2007

Additional Information

How to Cite

Kakinuma, Y., Iida, H., Sekizuka, T., Usui, K., Murayama, O., Takamiya, S., Millar, B., Moore, J. and Matsuda, M. (2007), Cloning, sequencing and characterization of a urease gene operon from urease-positive thermophilic Campylobacter (UPTC). Journal of Applied Microbiology, 103: 252–260. doi: 10.1111/j.1365-2672.2006.03212.x

Author Information

  1. 1

     Laboratory of Molecular Biology, School of Environmental Health Sciences, Azabu University, Sagamihara, Japan

  2. 2

     Department of Molecular and Cellular Parasitology, Juntendo University School of Medicine, Tokyo, Japan

  3. 3

     Department of Bacteriology, Northern Ireland Public Health Laboratory, Belfast City Hospital, Belfast, UK

*Motoo Matsuda, Laboratory of Molecular Biology, School of Environmental Health Sciences, Azabu University, Fuchinobe 1-17-71, Sagmihara 229-8501, Japan. E-mail: matsuda@azabu-u.ac.jp

Publication History

  1. Issue published online: 9 NOV 2006
  2. Article first published online: 9 NOV 2006
  3. 2005/1213: received 11 October 2005, revised 12 September 2006 and accepted 14 September 2006

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Keywords:

  • accessory genes;
  • overlap genes;
  • PCR;
  • structural genes;
  • TTG start codon;
  • urease operon;
  • urease-positive thermophilic Campylobacter

Abstract

Aims:  To clone, sequence and characterize the genetic organization of urease genes within urease-positive thermophilic Campylobacter (UPTC).

Methods and Results:  An approx. 5·1-kbp region encoding a urease gene operon was identified, when recombinant plasmid DNAs from a genomic DNA library of a Japanese isolate (CF89-12) of UPTC were analysed.

Conclusions:  Six closely spaced and putative open reading frames (ORFs) for ureA, ureB, ureE, ureF, ureG and ureH were detected. ATG codons initiated each ORF of the UPTC urease operon except for ureB and ureH, which commenced with the most probable TTG codon. Overlaps were detected between ureA and ureB and also between ureB and ureE. Probable ribosome-binding sites and a putative ρ-independent transcriptional termination region were identified. Two putative promoter structures, consisting of consensus sequences at the −35 like and −10 regions were also identified.

Significance and Impact of the Study:  Construction of a neighbour-joining tree based on the nucleotide sequence data of urease genes indicated that UPTC formed a cluster with some Helicobacter organisms separate from the other urease-producing bacteria, suggesting a commonly shared ancestry between UPTC and Helicobacter urease genes.

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