Avian- and mammalian-derived antibodies against adherence-associated proteins inhibit host cell colonization by Escherichia coli O157:H7
Article first published online: 17 APR 2007
Journal of Applied Microbiology
Volume 103, Issue 4, pages 1206–1219, October 2007
How to Cite
Cook, S.R., Maiti, P.K., DeVinney, R., Allen-Vercoe, E., Bach, S.J. and McAllister, T.A. (2007), Avian- and mammalian-derived antibodies against adherence-associated proteins inhibit host cell colonization by Escherichia coli O157:H7. Journal of Applied Microbiology, 103: 1206–1219. doi: 10.1111/j.1365-2672.2007.03334.x
- Issue published online: 17 APR 2007
- Article first published online: 17 APR 2007
- 2006/1554: received 7 November 2006, revised 18 January 2007 and accepted 31 January 2007
- avian IgY;
- enterohaemorrhagic Escherichia coli O157:H7;
- passive immunotherapy;
- polyclonal antibodies
Aim: To evaluate the potential for polyclonal antibodies targeting enterohaemorrhagic Escherichia coli (EHEC) virulence determinants to prevent colonization of host cells by E. coli O157:H7.
Methods and Results: Rats and laying hens were immunized with recombinant proteins from E. coli O157:H7, EspA, C-terminal intimin or EscF. Rat antisera (IgG) or chicken egg powders (IgY) were assessed for their ability to inhibit growth and colonization-associated processes of E. coli O157:H7. Mammalian antisera with antibodies to intimin, EspA or EscF effectively reduced adherence of the pathogen to HeLa cells (P < 0·05) and prevented type III secretion of Tir. Similarly, HeLa cells treated with chicken egg powder containing antibodies against intimin or EspA were protected from EHEC adherence (P < 0·05). Neither egg nor rat antibody preparations had any antibacterial effect on the growth of EHEC (P > 0·05).
Conclusions: Antibody preparations targeting EHEC adherence-associated factors were effective at preventing adhesion and intimate colonization-associated events.
Significance and Impact of the Study: This work indicates that immunotherapy with anti-adherence antibodies can reduce E. coli O157:H7 colonization of host cells. Passive immunization with specific antibodies may have the potential to reduce E. coli O157:H7 colonization in hosts such as cattle or humans.