Effect of gamma radiation on heat shock protein expression of four foodborne pathogens
Article first published online: 1 OCT 2008
© 2008 The Authors. Journal compilation © 2008 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 105, Issue 5, pages 1384–1391, November 2008
How to Cite
Caillet, S., Millette, M., Dussault, D., Shareck, F. and Lacroix, M. (2008), Effect of gamma radiation on heat shock protein expression of four foodborne pathogens. Journal of Applied Microbiology, 105: 1384–1391. doi: 10.1111/j.1365-2672.2008.03891.x
- Issue published online: 15 OCT 2008
- Article first published online: 1 OCT 2008
- 2007/1977: received 5 December 2007, revised 22 February 2008 and accepted 31 March 2008
- Escherichia coli;
- gamma radiation;
- heat shock proteins;
- Listeria monocytogenes;
- Salmonella Typhimurium;
- Staphylococcus aureus
Aims: The effects of gamma radiation on three heat shock proteins (Hsps) (GroEL, DnaK and GroES) synthesis in two Gram-negative (Escherichia coli and Salmonella serotype Typhimurium) and two Gram-positive (Staphylococcus aureus and Listeria monocytogenes) bacteria were investigated.
Methods and Results: The bacterial strains were treated with three radiation doses to induce cell damage, to obtain a viable but nonculturable state, and to cause cell death. Western blot analysis and quantification of Hsps in bacteria were performed immediately after irradiation treatment. In the four foodborne pathogens, GroEL was strongly induced by gamma rays in a dose-dependent manner, confirming the involvement of this protein in the cellular response to the stress generated by ionizing radiation. In addition, it was found that E. coli exposed to gamma radiation showed a significantly induction of DnaK and GroES proteins when compared with nonirradiated bacteria, whereas a GroES slight induction and a DnaK inhibition were observed in Salm. Typhimurium.
Conclusions: The gamma rays influence the synthesis of Hsps in foodborne pathogen in a way that critically depends on the radiation dose.
Significance and Impact of the Study: The study of stress response to several radiation doses was undertaken to elucidate how bacteria can survive in harsh conditions and cope with gamma radiation used to control foodborne pathogens and to characterize their adaptative response to this treatment.