Components derived from Pelargonium stimulate macrophage killing of Mycobacterium species
Article first published online: 21 JAN 2009
© 2009 The Authors. Journal compilation © 2009 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 106, Issue 4, pages 1184–1193, April 2009
How to Cite
Kim, C.E., Griffiths, W.J. and Taylor, P.W. (2009), Components derived from Pelargonium stimulate macrophage killing of Mycobacterium species. Journal of Applied Microbiology, 106: 1184–1193. doi: 10.1111/j.1365-2672.2008.04085.x
- Issue published online: 9 MAR 2009
- Article first published online: 21 JAN 2009
- 2008/1107: received 30 June 2008, revised 3 September 2008 and accepted 22 September 2008
- Mycobacterium tuberculosis;
- Mycobacterium fortuitum;
- Pelargonium reniforme;
Aims: To determine the capacity of extracts of Pelargonium reniforme and Pelargonium sidoides, plants of the Geraniaceae family, to stimulate the uptake and killing of mycobacteria by murine macrophages and to identify the constituents that are responsible.
Methods and results: Bioassay-guided fractionation of aqueous P. reniforme extracts yielded five chemically distinct structures with the capacity to increase the rate of intracellular killing by macrophages. These were: gallic acid, methyl gallate, myricetin and quercitin-3-O-β-d-glucoside, in addition to the previously unrecognized constituent 1-O-(2-(4-methoxyphenyl)ethyl-6-O-galloyl-glucopyranoside. Kinetics of intracellular accumulation of Mycobacterium tuberculosis and Mycobacterium fortuitum by macrophages were indistinguishable; pure preparations of the four previously known plant constituents stimulated macrophage killing, but not uptake, of M. tuberculosis and M. fortuitum equally well.
Conclusions: A number of distinct molecular species are present in the medicinal plant P. reniforme that stimulate the killing of the intracellular pathogen M. tuberculosis.
Significance and Impact of the Study: These observations support the view that Pelargonium extracts may have utility in the treatment of tuberculosis.