Identification of a new adhesin-like protein from Lactobacillus mucosae ME-340 with specific affinity to the human blood group A and B antigens
Article first published online: 13 MAR 2010
© 2010 The Authors. Journal compilation © 2010 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 109, Issue 3, pages 927–935, September 2010
How to Cite
Watanabe, M., Kinoshita, H., Nitta, M., Yukishita, R., Kawai, Y., Kimura, K., Taketomo, N., Yamazaki, Y., Tateno, Y., Miura, K., Horii, A., Kitazawa, H. and Saito, T. (2010), Identification of a new adhesin-like protein from Lactobacillus mucosae ME-340 with specific affinity to the human blood group A and B antigens. Journal of Applied Microbiology, 109: 927–935. doi: 10.1111/j.1365-2672.2010.04719.x
- Issue published online: 16 AUG 2010
- Article first published online: 13 MAR 2010
- 2009/0745: received 26 April 2009, revised 17 February 2010 and accepted 8 March 2010
- ABC transporter;
- human blood group antigen;
Aims: To identify and characterize a new adhesin-like protein of probiotics that show specific adhesion to human blood group A and B antigens.
Methods and Results: Using the BIACORE assay, the adhesion of cell surface components obtained from four lactobacilli strains that adhered to blood group A and B antigens was tested. Their components showed a significant adhesion to A and B antigens when compared to the bovine serum albumin (BSA) control. The 1 mol l−1 GHCl fraction extracted from Lactobacillus mucosae ME-340 contained a 29-kDa band (Lam29) using SDS–PAGE. The N-terminal amino acid sequence and homology analysis showed that Lam29 was 90% similar to the substrate-binding protein of the ATP-binding cassette (ABC) transporter from Lactobacillus fermentum IFO 3956. The complete nucleotide sequence (858 bp) of Lam29 was determined and encoded a protein of 285 amino acid residues. Phylogenetic analysis and multiple sequence alignments indicated this protein may be related to the cysteine-binding transporter.
Conclusions: The adhesion of ME-340 strain to blood group A and B antigens was mediated by Lam29 that is a putative component of ABC transporter as an adhesin-like protein.
Significance and Impact of the Study: Lactobacillus mucosae ME-340 expressing Lam29 may be useful for competitive exclusion of pathogens via blood group antigen receptors in the human gastrointestinal mucosa and in the development of new probiotic foods.