Role of the probiotic strain Lactobacillus paracasei LMGP22043 carried by artichokes in influencing faecal bacteria and biochemical parameters in human subjects
Article first published online: 20 APR 2011
© 2011 The Authors. Journal of Applied Microbiology © 2011 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 111, Issue 1, pages 155–164, July 2011
How to Cite
Valerio, F., de Candia, S., Lonigro, S.L., Russo, F., Riezzo, G., Orlando, A., De Bellis, P., Sisto, A. and Lavermicocca, P. (2011), Role of the probiotic strain Lactobacillus paracasei LMGP22043 carried by artichokes in influencing faecal bacteria and biochemical parameters in human subjects. Journal of Applied Microbiology, 111: 155–164. doi: 10.1111/j.1365-2672.2011.05019.x
- Issue published online: 13 JUN 2011
- Article first published online: 20 APR 2011
- Accepted manuscript online: 29 MAR 2011 10:36AM EST
- 2011/0213: received 4 February 2011, revised 2 March 2011 and accepted 23 March 2011
- active food carrier;
- bacterial antagonism;
- faecal enzyme activity;
- Shannon diversity index;
- short-chain fatty acids
Aims: To evaluate the positive influence of the probiotic strain Lactobacillus paracasei LMGP22043 carried by artichokes into the human gut with special reference to faecal bacterial balance, short-chain fatty acid concentrations and enzyme activities in a randomized, double-blind human trial in comparison with probiotic-free artichokes (control).
Methods: Twenty subjects were randomized into two groups, which consumed daily 180 g of the artichoke product (probiotic or control) during two 15-day study periods (periods 1 and 2) separated by a 15-day washout in a crossover manner. Faecal samples were subjected to microbiological and biochemical analyses, and a strain-specific PCR was performed to monitor the probiotic strain.
Results: The probiotic strain, transported by the vegetable matrix, transiently colonized the gut of 17/20 subjects (median 6·87 log CFU g−1 faeces), antagonized Escherichia coli and Clostridium spp. and increased the genetic diversity of lactic population based on REP-PCR profiles, mainly after period 1.
Conclusions: The probiotic L. paracasei LMGP22043 successfully colonized the human gut and positively influenced faecal bacteria and biochemical parameters.
Significance and Impact of the Study: The association of the probiotic L. paracasei with a food carrier rich in fibre can represent a new strategy for favouring a daily supply of probiotics and attracting more consumers to vegetable food fortified with probiotic strains.