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Classification of Salmonella enterica serotypes from Australian poultry using repetitive sequence-based PCR
Article first published online: 1 NOV 2011
© 2011 The Authors. Journal of Applied Microbiology © 2011 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 112, Issue 1, pages 185–196, January 2012
How to Cite
Chenu, J.W., Cox, J.M. and Pavic, A. (2012), Classification of Salmonella enterica serotypes from Australian poultry using repetitive sequence-based PCR. Journal of Applied Microbiology, 112: 185–196. doi: 10.1111/j.1365-2672.2011.05172.x
- Issue published online: 13 DEC 2011
- Article first published online: 1 NOV 2011
- Accepted manuscript online: 10 OCT 2011 05:11AM EST
- 2011/1011: received 19 June 2011, revised 11 September 2011 and accepted 2 October 2011
- DiversiLab® System;
- molecular epidemiology;
Aims: To evaluate a semi-automated repetitive extragenic palindromic sequence-based PCR (rep-PCR) system for the classification of Salmonella serotypes from Australian poultry.
Methods and Results: Using a DNA fingerprint library within the DiversiLab® System, four separate databases were constructed (serogroup B, C, E and Other). These databases contained 483 serologically confirmed (reference laboratory) Salmonella isolates. A blinded set of Salmonella cultures (n = 155) were typed by rep-PCR, matched against the internal library and compared with traditional serotyping. The predicted (Kullback–Leibler) serotype of 143 (92·3%) isolates matched traditional typing (P < 0·05). Of the 12 (7·7%) remaining isolates, ten (6·5%) resulted in ‘No Match’, one (0·65%) was incorrectly matched to the library (Salm. subsp 1 ser 4,12:-:-), and the other (0·65%) was referenced as Salm. ser. Sofia, whereas rep-PCR and in-house serotyping concurred as Salmonella serovar Typhimurium. Financial analysis showed higher material cost (215%) and a lower labour component (47·5%) for rep-PCR compared with serotyping.
Conclusion: The DiversiLab® System, with serogroup databases, was successfully implemented as an adjunct for reference serotyping of Salmonella enterica.
Significance and Impact of the Study: The DiversiLab® System platform is a cost-effective and easy-to-use system, which can putatively determine Salmonella enterica serotypes within a few hours.