Adaptation of Pseudomonas aeruginosa to a pulsed light-induced stress
Article first published online: 24 JAN 2012
© 2011 No claim to French Government works. Journal of Applied Microbiology © 2011 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 112, Issue 3, pages 502–511, March 2012
How to Cite
Massier, S., Rincé, A., Maillot, O., Feuilloley, M.G.J., Orange, N. and Chevalier, S. (2012), Adaptation of Pseudomonas aeruginosa to a pulsed light-induced stress. Journal of Applied Microbiology, 112: 502–511. doi: 10.1111/j.1365-2672.2011.05224.x
- Issue published online: 9 FEB 2012
- Article first published online: 24 JAN 2012
- Accepted manuscript online: 21 DEC 2011 07:16PM EST
- 2011/1809: received 21 October 2011, revised 29 November 2011 and accepted 11 December 2011
- Pseudomonas aeruginosa;
- pulsed light technology;
- pulsed light-induced stress
Aims: Pulsed light (PL) technology is an efficient surface decontamination process. Used in low transmitted energy conditions, PL induces a stress that can be perceived by bacteria. The effect of such a PL stress was investigated on the highly environmental adaptable germ Pseudomonas aeruginosa PAO1.
Methods and Results: Pulses of transmitted energy (fluence) reaching 1·8 J cm−2 can kill 109 bacteria. Application of a lower sublethal PL dose allowed the bacteria to resist and survive more efficiently to a subsequent dose of PL. This sublethal dose was not increasing the mutation frequency of Ps. aeruginosa, but altered the abundance of 15 proteins as revealed by a global proteome analysis, including stress-induced proteins, phage-related proteins, energy and carbon metabolisms, cell motility, and transcription and translation regulators.
Conclusions: A response to a low-energy PL dose takes place in Ps. aeruginosa, reducing the energy conversion systems, while increasing transcription and translation processes to produce proteins involved in chaperone mechanisms and phage-related proteins, probably to protect the bacterium against a new PL-induced stress.
Significance and Impact of the Study: Taken together, these results suggest that a low-energy PL dose is sufficient to provoke adaptation of Ps. aeruginosa, leading to enhancing its resistance to a subsequent lethal treatment.