Arthrospira platensis biomass with high protein content cultivated in continuous process using urea as nitrogen source
Article first published online: 27 APR 2012
© 2012 The Authors. Journal of Applied Microbiology © 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 112, Issue 6, pages 1086–1094, June 2012
How to Cite
Avila-Leon, I., Chuei Matsudo, M., Sato, S. and de Carvalho, J.C.M. (2012), Arthrospira platensis biomass with high protein content cultivated in continuous process using urea as nitrogen source. Journal of Applied Microbiology, 112: 1086–1094. doi: 10.1111/j.1365-2672.2012.05303.x
- Issue published online: 10 MAY 2012
- Article first published online: 27 APR 2012
- Accepted manuscript online: 9 APR 2012 12:59AM EST
- 2011/1700: received 5 October 2011, revised 30 March 2012 and accepted 2 April 2012
- Arthrospira (Spirulina) platensis;
- biomass composition;
- continuous cultivation;
- dilution rate;
- kinetic parameters
Aims: Arthrospira platensis has been studied for single-cell protein production because of its biomass composition and its ability of growing in alternative media. This work evaluated the effects of different dilution rates (D) and urea concentrations (N0) on A. platensis continuous culture, in terms of growth, kinetic parameters, biomass composition and nitrogen removal.
Methods and results: Arthrospira platensis was continuously cultivated in a glass-made vertical column photobioreactor agitated with Rushton turbines. There were used different dilution rates (0·04–0·44 day−1) and urea concentrations (0·5 and 5 mmol l−1). With N0 = 5 mmol l−1, the maximum steady-state biomass concentration was1415 mg l−1, achieved with D = 0·04 day−1, but the highest protein content (71·9%) was obtained by applying D = 0·12 day−1, attaining a protein productivity of 106·41 mg l−1 day−1. Nitrogen removal reached 99% on steady-state conditions.
Conclusions: The best results were achieved by applying N0 = 5 mmol l−1; however, urea led to inhibitory conditions at D ≥ 0·16 day−1, inducing the system wash-out. The agitation afforded satisfactory mixture and did not harm the trichomes structure.
Significance and Impact of the Study: These results can enhance the basis for the continuous removal of nitrogenous wastewater pollutants using cyanobacteria, with an easily assembled photobioreactor.