Figure S1 Quantification of (A) ICA and SSC, (B) UCP, and (C) Sketa in presence of various concentrations of Enterococcus. Error bars represent standard deviation.

Table S1. Thermal conditions for the five qPCR methods.

Table S2. Primer and probe sequences. All sequences for the ScorpionN method are proprietary and not listed here.

Table S3. Standard curves for all qPCR assays*

Table S4. Internal control Ct values for uninhibited reference materials.

Table S5. Enterococcus concentration (calibrator cell equivalent per 100ml) by qPCR from selected environmental water samples, based on qPCR screening results prior to inhibition analysis.

Appendix S1. An example of using simulation to assess impact of Enterococcus qPCR inhibition in ambient water monitoring

Appendix S2. Standard Operating Procedure for a spiking-followed-by-dilution approach to detect inhibition for qPCR assays

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