Characterization and emulsifying property of a novel bioemulsifier by Aeribacillus pallidus YM-1
Article first published online: 10 MAY 2012
© 2012 The Authors. Journal of Applied Microbiology © 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 113, Issue 1, pages 44–51, July 2012
How to Cite
Zheng, C., Li, Z., Su, J., Zhang, R., Liu, C. and Zhao, M. (2012), Characterization and emulsifying property of a novel bioemulsifier by Aeribacillus pallidus YM-1. Journal of Applied Microbiology, 113: 44–51. doi: 10.1111/j.1365-2672.2012.05313.x
- Issue published online: 13 JUN 2012
- Article first published online: 10 MAY 2012
- Accepted manuscript online: 19 APR 2012 10:24AM EST
- 2011/2205: received 28 December 2011, revised 15 March 2012 and accepted 13 April 2012
- Aeribacillus pallidus;
- emulsification index;
Aims: Biosurfactants and bioemulsifiers commonly have the advantages of biodegradability, low toxicity, selectivity and biocompatibility over chemically synthesized surfactants. The goal of the study is to present a novel bioemulsifier with great application potential.
Methods and Results: Aeribacillus pallidus YM-1, isolated from crude oil contaminated soil, was found to produce a novel high molecular bioemulsifier with an emulsification index of 60 ± 1% without remarkable surface tension reduction (45·7 ± 0·1 mN m−1). The number-average molecular weight was determined as 526 369 Da by gel permeation chromatography analysis. Bioemulsifier was subjected to FT-IR and a complex of carbohydrates (41·1%), lipids (47·6%) and proteins (11·3%) was determined.
Conclusions: The bioemulsifier of A. pallidus YM-1 was isolated from the glucose-based culture medium and characterized with the help of chemical analytical techniques. The bioemulsifier exhibited a promising emulsifying property for biotechnology application potential in bioremediation and microbial enhanced oil recovery.
Significance and Impact of the Study: This is the first report of the bioemulsifier production by A. pallidus. The potential emulsifying activity of the bioemulsifier in the present study may be explored in various biotechnological and industrial applications.