This research was developed in the laboratories of the Veterinary Department, Viçosa Federal University, and in the Pharmaceutical Sciences Faculty, São Paulo University.
Bacteriocinogenic and virulence potential of Enterococcus isolates obtained from raw milk and cheese
Article first published online: 11 JUN 2012
© 2012 The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 113, Issue 2, pages 318–328, August 2012
How to Cite
Moraes, P.M., Perin, L.M., Todorov, S.D., Silva, A., Franco, B.D.G.M. and Nero, L.A. (2012), Bacteriocinogenic and virulence potential of Enterococcus isolates obtained from raw milk and cheese. Journal of Applied Microbiology, 113: 318–328. doi: 10.1111/j.1365-2672.2012.05341.x
- Issue published online: 16 JUL 2012
- Article first published online: 11 JUN 2012
- Accepted manuscript online: 15 MAY 2012 10:27AM EST
- Manuscript Accepted: 9 MAY 2012
- Manuscript Revised: 24 APR 2012
- Manuscript Received: 30 JAN 2012
Vol. 113, Issue 6, 1564, Article first published online: 16 NOV 2012
To provide molecular and phenotypical characterization of Enterococcus isolates obtained from raw milk and cheese, regarding their bacteriocinogenic and virulence activity.
Methods and Results
Forty-three bacteriocinogenic enterococci isolates were identified by 16s rDNA, fingerprinted by RAPD-PCR analysis and tested by PCR for the presence of genes for lantibiotics (lanM, lanB and lanC) and enterocins (entA, entB, entP, entL50AB and entAS48) and by phenotypical methods for bacteriocin production and inhibitory spectrum. Also, the virulence of the isolates was evaluated by PCR for genes gelE, hyl, asa1, esp, cylA, efaA, ace, vanA, vanB, hdc1, hdc2, tdc and odc and by phenotypical tests for gelatinase, lipase, DNAse and α- and β-haemolysis. Most isolates (93·0%) harboured at least one lantibiotic or enterocin gene and were positive for several tested virulence genes, mainly asa1 (100%), gelE (93·0%) and efaA (83·7%). 53·5% of the isolates presented β-haemolysis.
Enterococcus spp. isolates presented an interesting potential application for food preservation because of bacteriocin production; however, virulence-related genes were identified in all RAPD profiles.
Significance and Impact of the Study
The study demonstrated the contradictory characteristics of the tested Enterococcus isolates: they presented a good potential for application in food biopreservation but contained several virulence factors.