Investigation of the impact of feeding Lactobacillus plantarum CRL 1815 encapsulated in microbially derived polymers on the rat faecal microbiota
Article first published online: 29 JUN 2012
© 2012 The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 113, Issue 2, pages 399–410, August 2012
How to Cite
Jiménez-Pranteda, M.L., Aguilera, M., McCartney, A.L., Hoyles, L., Jiménez-Valera, M., Náder-Macías, M.E., Ramos-Cormenzana, A. and Monteoliva-Sánchez, M. (2012), Investigation of the impact of feeding Lactobacillus plantarum CRL 1815 encapsulated in microbially derived polymers on the rat faecal microbiota. Journal of Applied Microbiology, 113: 399–410. doi: 10.1111/j.1365-2672.2012.05343.x
- Issue published online: 16 JUL 2012
- Article first published online: 29 JUN 2012
- Accepted manuscript online: 21 MAY 2012 07:55AM EST
- Manuscript Accepted: 11 MAY 2012
- Manuscript Revised: 18 APR 2012
- Manuscript Received: 16 FEB 2012
- fluorescence in situ hybridization;
- temporal temperature gradient gel electrophoresis
The aim of this study was to evaluate the impact of the administration of microencapsulated Lactobacillus plantarum CRL 1815 with two combinations of microbially derived polysaccharides, xanthan : gellan gum (1%:0·75%) and jamilan : gellan gum (1%:1%), on the rat faecal microbiota.
Methods and Results
A 10-day feeding study was performed for each polymer combination in groups of 16 rats fed either with placebo capsules, free or encapsulated Lact. plantarum or water. The composition of the faecal microbiota was analysed by fluorescence in situ hybridization and temporal temperature gradient gel electrophoresis. Degradation of placebo capsules was detected, with increased levels of polysaccharide-degrading bacteria. Xanthan : gellan gum capsules were shown to reduce the Bifidobacterium population and increase the Clostridium histolyticum group levels, but not jamilan : gellan gum capsules. Only after administration of jamilan : gellan gum-probiotic capsules was detected a significant increase in Lactobacillus-Enterococcus group levels compared to controls (capsules and probiotic) as well as two bands were identified as Lact. plantarum in two profiles of ileum samples.
Exopolysaccharides constitute an interesting approach for colon-targeted delivery of probiotics, where jamilan : gellan gum capsules present better biocompatibility and promising results as a probiotic carrier.
Significance and Impact of Study
This study introduces and highlights the importance of biological compatibility in the encapsulating material election, as they can modulate the gut microbiota by themselves, and the use of bacterial exopolysaccharides as a powerful source of new targeted-delivery coating material.