Bacteriophage control of vancomycin-resistant enterococci in cattle compost
Article first published online: 12 JUL 2012
© 2012 The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 113, Issue 3, pages 499–507, September 2012
How to Cite
Otawa, K., Hirakata, Y., Kaku, M. and Nakai, Y. (2012), Bacteriophage control of vancomycin-resistant enterococci in cattle compost. Journal of Applied Microbiology, 113: 499–507. doi: 10.1111/j.1365-2672.2012.05361.x
- Issue published online: 14 AUG 2012
- Article first published online: 12 JUL 2012
- Accepted manuscript online: 15 JUN 2012 11:32PM EST
- Manuscript Accepted: 9 JUN 2012
- Manuscript Revised: 31 MAY 2012
- Manuscript Received: 6 APR 2012
- Enterococcus faecium phage;
- phage control;
- vancomycin-resistant enterococci
To isolate bacteriophage that infects vancomycin-resistant enterococci (VRE) and to investigate the ability of this phage to diminish VRE number in vitro and in experimentally VRE-inoculated compost.
Methods and Results
We sampled 106 solid or water samples, including 101 bovine faecal samples; lytic phage named Vrep-5 was isolated from one bovine faecal sample by plaque assay using the clinical VRE isolate FN1 (Enterococcus faecium). Vrep-5 generated clear plaques 1 mm in diameter and exhibited characteristics of the family Myoviridae A1, with a spherical head (122 ± 16 nm) and a contractile tail (152 ± 17 nm long). Vrep-5 lysed other bacterial strains, including Enterococcus faecalis. Inoculation of vrep-5 into 0·5 g unsterilized compost experimentally inoculated with FN1 at the multiplicity of infection of 1500 (8·8 × 104 CFU g−1 VRE and 1·3 × 108 PFU g−1 vrep-5) led to a decrease of >3 log10 in VRE abundance compared with the untreated control after 24 h of incubation.
The data show that bacteriophage vrep-5 is effective in the rapid reduction in VRE colonization in compost.
Significance and Impact of the Study
The present study gives valuable new knowledge in the fight against VRE in the animal production.