Variability in the concentrations of volatile metabolites emitted by genotypically different strains of Pseudomonas aeruginosa

Authors

  • V. Shestivska,

    1. J. Heyrovsky Institute of Physical Chemistry of Science, Academy of Science of the Czech Republic, Prague, Czech Republic
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  • P. Španěl,

    1. J. Heyrovsky Institute of Physical Chemistry of Science, Academy of Science of the Czech Republic, Prague, Czech Republic
    2. Institute for Science and Technology in Medicine, School of Medicine, University of Keele, Stoke on Trent, UK
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  • K. Dryahina,

    1. J. Heyrovsky Institute of Physical Chemistry of Science, Academy of Science of the Czech Republic, Prague, Czech Republic
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  • K. Sovová,

    1. J. Heyrovsky Institute of Physical Chemistry of Science, Academy of Science of the Czech Republic, Prague, Czech Republic
    2. Department of Physical Macromolecular Chemistry, Faculty of Science, Charles University, Prague, Czech Republic
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  • D. Smith,

    1. Institute for Science and Technology in Medicine, School of Medicine, University of Keele, Stoke on Trent, UK
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  • M. Musílek,

    1. Centre of Epidemiology and Microbiology, National Institute of Public Health, Prague, Czech Republic
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  • A. Nemec

    Corresponding author
    1. Centre of Epidemiology and Microbiology, National Institute of Public Health, Prague, Czech Republic
    • J. Heyrovsky Institute of Physical Chemistry of Science, Academy of Science of the Czech Republic, Prague, Czech Republic
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Correspondence

Alexandr Nemec, Laboratory of Bacterial Genetics, Centre of Epidemiology and Microbiology, National Institute of Public Health, Srobárova 48, 10042 Prague, Czech Republic. E-mail: anemec@szu.cz

Abstract

Aims

To characterize the volatile metabolites produced by genotypically diverse strains of Pseudomonas aeruginosa in order to evaluate their potential for use as biomarkers of lung infection in noninvasive breath analysis.

Methods and Results

Volatile organic compounds (VOCs) emitted from 36 clinical strains of Ps. aeruginosa (belonging to different multilocus sequence types) cultured in liquid and on solid media were analysed by gas chromatography mass spectrometry (GC-MS) and selected ion flow tube mass spectrometry (SIFT-MS). Several previously identified VOCs were detected, including ethanol, acetone, 2-butanone, 2-pentanone, isoprene, aminoacetophenone, dimethyl sulphide, dimethyl disulphide, dimethyl trisulphide and methyl thiocyanate. Additionally, significant production of 3-methyl-butanone, acetophenone, methylthioacetate and methyl thiobutanoate was observed for the first time in this study. SIFT-MS quantifications of VOCs showed high variability between genotypically distinct strains.

Conclusions

The data obtained indicate that the production rates of the volatile biomarkers of Ps. aeruginosa vary by two orders of magnitude between different strains cultured under the same conditions. Similar variability was observed for both liquid and solid media.

Significance and Impact of the Study

Inter-strain genotypic variability strongly influences the concentrations of the volatile biomarkers from Ps. aeruginosa. A group of several biomarkers quantified in real time in exhaled breath may thus provide a more valuable indicator of the course of pulmonary infections compared to a single biomarker.

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