Variability in the concentrations of volatile metabolites emitted by genotypically different strains of Pseudomonas aeruginosa
Article first published online: 24 JUL 2012
© 2012 The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 113, Issue 3, pages 701–713, September 2012
How to Cite
Shestivska, V., Španěl, P., Dryahina, K., Sovová, K., Smith, D., Musílek, M. and Nemec, A. (2012), Variability in the concentrations of volatile metabolites emitted by genotypically different strains of Pseudomonas aeruginosa . Journal of Applied Microbiology, 113: 701–713. doi: 10.1111/j.1365-2672.2012.05370.x
- Issue published online: 14 AUG 2012
- Article first published online: 24 JUL 2012
- Accepted manuscript online: 24 JUN 2012 09:43PM EST
- Manuscript Revised: 20 JUN 2012
- Manuscript Accepted: 20 JUN 2012
- Manuscript Received: 3 APR 2012
- Czech Science Foundation (GACR)
- breath analysis;
- cystic fibrosis;
- gas chromatography mass spectrometry;
- mass spectrometry;
- multilocus sequence typing;
- selected ion flow tube mass spectrometry
To characterize the volatile metabolites produced by genotypically diverse strains of Pseudomonas aeruginosa in order to evaluate their potential for use as biomarkers of lung infection in noninvasive breath analysis.
Methods and Results
Volatile organic compounds (VOCs) emitted from 36 clinical strains of Ps. aeruginosa (belonging to different multilocus sequence types) cultured in liquid and on solid media were analysed by gas chromatography mass spectrometry (GC-MS) and selected ion flow tube mass spectrometry (SIFT-MS). Several previously identified VOCs were detected, including ethanol, acetone, 2-butanone, 2-pentanone, isoprene, aminoacetophenone, dimethyl sulphide, dimethyl disulphide, dimethyl trisulphide and methyl thiocyanate. Additionally, significant production of 3-methyl-butanone, acetophenone, methylthioacetate and methyl thiobutanoate was observed for the first time in this study. SIFT-MS quantifications of VOCs showed high variability between genotypically distinct strains.
The data obtained indicate that the production rates of the volatile biomarkers of Ps. aeruginosa vary by two orders of magnitude between different strains cultured under the same conditions. Similar variability was observed for both liquid and solid media.
Significance and Impact of the Study
Inter-strain genotypic variability strongly influences the concentrations of the volatile biomarkers from Ps. aeruginosa. A group of several biomarkers quantified in real time in exhaled breath may thus provide a more valuable indicator of the course of pulmonary infections compared to a single biomarker.