Detection of Shiga toxin variants among Shiga toxin–forming Escherichia coli isolates from animal stool, meat and human stool samples in India
Article first published online: 21 AUG 2012
© 2012 The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 113, Issue 5, pages 1208–1216, November 2012
How to Cite
Kumar, A., Taneja, N., Kumar, Y. and Sharma, M. (2012), Detection of Shiga toxin variants among Shiga toxin–forming Escherichia coli isolates from animal stool, meat and human stool samples in India. Journal of Applied Microbiology, 113: 1208–1216. doi: 10.1111/j.1365-2672.2012.05415.x
- Issue published online: 17 OCT 2012
- Article first published online: 21 AUG 2012
- Accepted manuscript online: 26 JUL 2012 03:22AM EST
- Manuscript Accepted: 21 JUL 2012
- Manuscript Revised: 17 JUL 2012
- Manuscript Received: 1 MAY 2012
- Indian Council of Medical Research (ICMR). Grant Number: 5/8-1213-D/2006 ECD-II
- animal stool;
- human stool samples;
- HUS ;
- PCR ;
- Shiga toxin variants
To study the prevalence and distribution of various variants in the stx gene of Shiga toxin–producing Escherichia coli (STEC) isolated from diverse environmental sources (animal stool, meat) and human illness, from a large geographic area in India, and to understand the association between variants, serotype distribution and human disease.
Methods and Results
A surveillance for STEC was conducted in the semi-urban and rural areas of Punjab, Himachal, Haryana and Chandigarh. Shiga toxin–producing Escherichia coli isolates (80 animal stool, 39 meat, 21 human stool from diarrhoea and HUS cases) were characterized for stx variants by PCR. Shiga-like toxin (Stx) was detected using Ridascreen-EIA assay. Variant stx2c was the most common (25·1%), followed by stx1d (13%), stx1c (10·7%) and stx2d (9·2%), whereas stx2e, stx2f and stx2g were absent. Only 8/21 (38%) human isolates harboured stx variants, of which stx2c and stx2d were found in 2 and 1 isolates, respectively. The low frequency of carriage of these potentially more pathogenic variants may explain the low severity of human illness seen in India. Shiga-like toxin was detected in only 42 of the isolates positive for the stx genes probably due to the low levels of toxins produced. Serogroup distribution was found to be diverse, suggesting the lack of any predominant circulating type.
The presence of stx variants 1c, 1d, 2c and stx2d in diverse environmental and human sources in India was demonstrated. The prevalence of the most common subtype stx2c found in this study in animal isolates may pose a threat to the public health. We report the subtyping of human STEC isolates and report the presence of stx1d subtype for the first time from India.
Significance and Impact of the Study
We demonstrated the presence of potentially pathogenic subtypes in the environmental specimens which may act as a reservoir for human infections. Serogroups new to India were also reported.