Screening of biofilm formation by beneficial vaginal lactobacilli and influence of culture media components
Article first published online: 7 SEP 2012
© 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 113, Issue 6, pages 1517–1529, December 2012
How to Cite
Terraf, M.C. L., Juárez Tomás, M.S., Nader-Macías, M.E.F. and Silva, C. (2012), Screening of biofilm formation by beneficial vaginal lactobacilli and influence of culture media components. Journal of Applied Microbiology, 113: 1517–1529. doi: 10.1111/j.1365-2672.2012.05429.x
- Issue published online: 16 NOV 2012
- Article first published online: 7 SEP 2012
- Accepted manuscript online: 16 AUG 2012 06:46AM EST
- Manuscript Accepted: 3 AUG 2012
- Manuscript Revised: 31 JUL 2012
- Manuscript Received: 20 JUN 2012
- Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina (CONICET). Grant Number: PIP 632
- Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT). Grant Number: PICT 2007-543
- culture medium components;
- microbial growth;
- Tween 80
To assess the ability of vaginal lactobacilli to form biofilm under different culture conditions and to determine the relationship between their growth and the capability of biofilm formation by selected strains.
Methods and Results
Fifteen Lactobacillus strains from human vagina were tested for biofilm formation by crystal violet staining. Only Lactobacillus rhamnosus Centro de Referencia para Lactobacilos Culture Collection (CRL) 1332, Lact. reuteri CRL 1324 and Lact. delbrueckii CRL 1510 were able to grow and form biofilm in culture media without Tween 80. However, Lact. gasseri CRL 1263 (a non-biofilm-forming strain) did not grow in these media. Scanning electron microscopy showed that Lact. rhamnosus CRL 1332 and Lact. reuteri CRL 1324 formed a highly structured biofilm, but only Lact. reuteri CRL 1324 showed a high amount of extracellular material in medium without Tween.
Biofilm formation was significantly influenced by the strain, culture medium, inoculum concentration, microbial growth and chemical nature of the support used for the assay.
Significance and Impacts of the Study
The results allow the selection of biofilm-forming vaginal Lactobacillus strains and the conditions and factors that affect this phenomenon.