M.X.L. and H.Y.Z. contributed equally to this work.
Production of secoisolariciresinol from defatted flaxseed by bacterial biotransformation
Article first published online: 19 SEP 2012
© 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 113, Issue 6, pages 1352–1361, December 2012
How to Cite
Li, M.-X., Zhu, H.-Y., Yang, D.-H., Ma, X.-Q., Wang, C.-Z., Cai, S.-Q., Liu, G.-R., Ku, B.-S. and Liu, S.-L. (2012), Production of secoisolariciresinol from defatted flaxseed by bacterial biotransformation. Journal of Applied Microbiology, 113: 1352–1361. doi: 10.1111/j.1365-2672.2012.05436.x
- Issue published online: 16 NOV 2012
- Article first published online: 19 SEP 2012
- Accepted manuscript online: 24 AUG 2012 12:08AM EST
- Manuscript Accepted: 17 AUG 2012
- Manuscript Revised: 3 AUG 2012
- Manuscript Received: 30 MAY 2012
- National Natural Science Foundation of China. Grant Number: 30672622
- National Science and Technology Major Projects for Major New Drugs Innovation and Development. Grant Number: 2011ZX09102-011
- NSFC. Grant Number: 30970078
- Natural Science Foundation of Heilongjiang Province of China
- NSFC. Grant Numbers: 30370774, 30870098, 30970119
- Peking University Health Science Center
- Bacteroides uniformis;
- intestinal bacteria;
- secoisolariciresinol diglucoside
Secoisolariciresinol (SECO) is increasingly recognized for potential clinical application because of its preventive effects against breast and colon cancers, atherosclerosis and diabetes, and its production through biotransformation has been attempted. However, previously reported bacteria all required stringent anaerobic culture conditions, precluding large-scale production. Here, we report the isolation and characterization of bacteria that produce SECO under less stringent anaerobic culture conditions.
Methods and Results
Using defatted flaxseed as raw material, we isolated a facultative anaerobic bacterium from human faeces that hydrolysed secoisolariciresinol diglucoside-3-hydroxy-3-methyl glutaric acid (SDG-HMGA) oligomers in flaxseed to produce SECO. Both conventional assays and 16S rRNA gene sequence analysis demonstrated its close relatedness with Bacteroides uniformis. The transformation efficiency of SDG in defatted flaxseed to SECO was more than 80% by this bacterial strain. We investigated factors that might influence fermentation, such as redox potential and pH, for large-scale fermentation of defatted flaxseed to produce SECO.
The method to produce SECO through biotransformation of defatted flaxseed with this bacterial strain is highly efficient and economic.
Significance and Impact of the Study
This bacterial strain can transform SDG to SECO under less stringent anaerobic culture conditions, which will greatly facilitate industry-scale production of SECO.