Lactobacillus pentosus var. plantarum C29 protects scopolamine-induced memory deficit in mice
Article first published online: 17 SEP 2012
© 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 113, Issue 6, pages 1498–1506, December 2012
How to Cite
Jung, I.-H., Jung, M.-A., Kim, E.-J., Han, M.J. and Kim, D.-H. (2012), Lactobacillus pentosus var. plantarum C29 protects scopolamine-induced memory deficit in mice. Journal of Applied Microbiology, 113: 1498–1506. doi: 10.1111/j.1365-2672.2012.05437.x
- Issue published online: 16 NOV 2012
- Article first published online: 17 SEP 2012
- Accepted manuscript online: 24 AUG 2012 03:12AM EST
- Manuscript Accepted: 14 AUG 2012
- Manuscript Revised: 13 AUG 2012
- Manuscript Received: 11 JUN 2012
- lactic acid bacteria;
- Lactobacillus pentosus var. plantarum;
- memory deficit-protecting effect
In the preliminary study, kimchi, a traditional food fermented with Chinese cabbage, protected scopolamine-induced mouse memory deficit in passive avoidance test. Therefore, we screened protective ingredients, particularly lactic acid bacteria, from Chinese cabbage kimchi against scopolamine-induced memory deficit in mice.
Methods and Results
Lactic acid bacteria, isolated from Chinese cabbage kimchi, were identified by 16S rDNA sequence analysis, G+C content and cellular fatty acid composition and sugar fermentation test. Memory deficit was induced in mice by intraperitoneally injecting with scopolamine.
Kimchi, particularly its supernatant, protected scopolamine-induced memory deficit in mice in passive avoidance test. Of kimchi ingredients, a lactic acid bacterium, strain C29, potently protected scopolamine-induced memory deficit in mice. C29 was a gram-positive, catalase-negative, anaerobic and non-motile rod. Its pylogenetic property was near to Lactobacillus pentosus (99%) and Lact. plantarum (99%). However, C29 fermented inulin and L-rhamnose and grew in pH 3 and at 45°C in contrast with Lact. pentosus and Lact. plantarum. Therefore, it named to be Lact pentosus var. plantarum C29. The strain C29 protected scopolamine-induced memory deficit in Y-maze and Morris water maze tests. Furthermore, C29 increased hippocampal BDNF and p-CREB expressions, which were reduced by scopolamine.
Lactobacillus pentosus var. plantarum C29 may protect memory deficit by inducing BDNF and p-CREB expressions.
Significance and Impact of the Study
Lactic acid bacteria, such as Lact pentosus var. plantarum C29, may prevent memory deficit and its contained fermented foods may be beneficial for dementia.