Appendix S1 Putative highland species or species pairs cited by Martin (1958) with disjunct distributions in the highlands of the Sierra Made Occidental and Sierra Madre Oriental, and either absent or replaced by sister taxa along the Transvolcanic Belt.

Appendix S2 (a) Collection and voucher data for genetic samples of highland rattlesnakes of the Crotalus intermedius group used in this study and deposited in the Royal Ontario Museum (ROM), and (b) GenBank accession numbers for outgroup samples used.

Appendix S3 Map showing geo-referenced localities of Crotalus pricei samples used in species distribution models in this study.

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