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Appendix S1 List of specimens included in the present study.

Appendix S2 (a) List of primers used in the present study; (b) regions of uncertain homology assessment.

Appendix S3 Topology and area coding matrix used for ancestral area reconstruction.

Appendix S4 Bayesian phylogeny of subgenus Tildenia showing all terminal species and accessions as listed in Appendix S1.

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