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Appendix S1 Methods used for divergence timeestimation (IMa).

Appendix S2 (a) Diversity statistics(Watterson’s θ; nucleotide diversity, π) bypopulation and species. (b) Tamura–Nei population averagepairwise distances (dA) for adjacent populationscrossing the boundaries constructed with barrier. (c) Results fromhierarchical AMOVAs for each species.

Appendix S3 barrier figure forBursera simaruba from non-bootstrapped dA values.

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