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Disrupted phylogeographical microsatellite and chloroplast DNA patterns indicate a vicariance rather than long-distance dispersal origin for the disjunct distribution of the Chilean endemic Dioscorea biloba (Dioscoreaceae) around the Atacama Desert

Authors

  • Juan Viruel,

    1. Departamento de Agricultura y Economía Agraria, Escuela Politécnica Superior de Huesca, Universidad de Zaragoza, C/Carretera de Cuarte Km 1, E22071, Huesca, Spain
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  • Pilar Catalán,

    1. Departamento de Agricultura y Economía Agraria, Escuela Politécnica Superior de Huesca, Universidad de Zaragoza, C/Carretera de Cuarte Km 1, E22071, Huesca, Spain
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  • José Gabriel Segarra-Moragues

    Corresponding author
    1. Centro de Investigaciones sobre Desertificación (CIDE-CSIC-UV-GV), C/Carretera Moncada-Náquera Km 4.5, E-46113, Moncada, Valencia, Spain
      José Gabriel Segarra-Moragues, Centro de Investigaciones sobre Desertificación (CIDE-CSIC-UV-GV), C/Moncada-Náquera Km 4.5, E-46113, Moncada, Valencia, Spain.
      E-mail: j.gabriel.segarra@uv.es
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José Gabriel Segarra-Moragues, Centro de Investigaciones sobre Desertificación (CIDE-CSIC-UV-GV), C/Moncada-Náquera Km 4.5, E-46113, Moncada, Valencia, Spain.
E-mail: j.gabriel.segarra@uv.es

Abstract

Aim  The Chilean endemic Dioscorea biloba (Dioscoreaceae) is a dioecious geophyte that shows a remarkable 600 km north–south disjunction in the peripheral arid area of the Atacama Desert. Its restricted present-day distribution and probable Neogene origin indicate that its populations have a history linked to that of the Atacama Desert, making this an ideal model species with which to investigate the biogeography of the region.

Location  Chile, Atacama Desert and peripheral arid area.

Methods  Two hundred and seventy-five individuals from nine populations were genotyped for seven nuclear microsatellite loci, and plastid trnL–F and trnT–L sequences were obtained for a representative subset of these. Analyses included the estimation of genetic diversity and population structure through clustering, Bayesian and analysis of molecular variance analyses, and statistical parsimony networks of chloroplast haplotypes. Isolation by distance was tested against alternative dispersal hypotheses.

Results  Microsatellite markers revealed moderate to high levels of genetic diversity within populations, with those from the southern Limarí Valley showing the highest values and northern populations showing less exclusive alleles. Bayesian analysis of microsatellite data identified three genetic groups that corresponded to geographical ranges. Chloroplast phylogeography revealed no haplotypes shared between northern and southern ranges, and little haplotype sharing between the two neighbouring southern valleys. Dispersal models suggested the presence of extinct hypothetical populations between the southern and northern ranges.

Main conclusions  Our results are consistent with prolonged isolation of the northern and southern groups, mediated by the life-history traits of the species. Significant isolation was revealed at both large and moderate distances as gene flow was not evident even between neighbouring valleys. Bayesian analyses of microsatellite and chloroplast haplotype diversity identified the southern area of Limarí as the probable area of origin of the species. Our data do not support recent dispersal of D. biloba from the southern range into Antofagasta, but indicate the fragmentation of an earlier wider range, concomitant with the Pliocene–Pleistocene climatic oscillations, with subsequent extinctions of the Atacama Desert populations and the divergence of the peripheral ones as a consequence of genetic drift.

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