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Genetic polymorphism of CYP2C8 in three Malaysian ethnics: CYP2C8*2 and CYP2C8*3 are found in Malaysian Indians
Article first published online: 5 SEP 2005
DOI: 10.1111/j.1365-2710.2005.00653.x
Additional Information
How to Cite
Muthiah, Y. D., Lee, W. L., Teh, L. K., Ong, C. E. and Ismail, R. (2005), Genetic polymorphism of CYP2C8 in three Malaysian ethnics: CYP2C8*2 and CYP2C8*3 are found in Malaysian Indians. Journal of Clinical Pharmacy and Therapeutics, 30: 487–490. doi: 10.1111/j.1365-2710.2005.00653.x
Publication History
- Issue published online: 5 SEP 2005
- Article first published online: 5 SEP 2005
- Received 25 April 2004, Accepted 15 March 2005
- Abstract
- Article
- References
- Cited By
Keywords:
- allele frequency;
- CYP2C8;
- polymorphism
Summary
Background: CYP2C8 is genetically polymorphic. Four variants, CYP2C8*2, CYP2C8*3, CYP2C8*4 and CYP2C8*5, which contain mutations in the coding regions have been reported to exhibit different enzyme activity as compared with CYP2C8*1.
Objective: To determine the allele frequency of three codon-changing variants (CYP2C8*2, CYP2C8*3 and CYP2C8*4) in the Malaysian population.
Method: Healthy unrelated volunteers from three major races in Malaysia were recruited. The study was approved by the local Research Ethics Committee. DNA was extracted using a standard protocol. A two-step multiplex PCR method was developed to detect three alleles of CYP2C8. PCR results were confirmed by subsequent direct DNA sequencing.
Result: Only the Indians showed CYP2C8 polymorphism with allele frequency of 98% for CYP2C8*1, 0·8% for CYP2C8*2 and 1·2% for CYP2C8*3. CYP2C8*4 was not detected in any of the ethnic groups.
Conclusion: To the best of our knowledge, the current study described, for the first time polymorphisms of CYP2C8 in Malaysian Indians.