Genetic polymorphism of CYP2C8 in three Malaysian ethnics: CYP2C8*2 and CYP2C8*3 are found in Malaysian Indians

Authors

  • Y. D. Muthiah PhD candidate,

    1. Pharmacogenetics Study Group, Institute for Research in Molecular Medicine and Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, Kelantan
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  • W. L. Lee PhD candidate,

    1. Pharmacogenetics Study Group, Institute for Research in Molecular Medicine and Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, Kelantan
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  • L. K. Teh PhD,

    1. Pharmacogenetics Study Group, Institute for Research in Molecular Medicine and Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, Kelantan
    2. Faculty of Pharmacy, Universiti Teknologi Mara, Selangor
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  • C. E. Ong PhD,

    1. Department of Pharmacy, International Medical University, Kuala Lumpur, Malaysia
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  • R. Ismail PharmD

    1. Pharmacogenetics Study Group, Institute for Research in Molecular Medicine and Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, Kelantan
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Rusli Ismail, Associate Professor, Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 16150 Kota Bharu, Kelantan, Malaysia. Tel.: 609 7664 922; fax: 609 7653370; e-mail: isrusli@kb.usm.my

Summary

Background: CYP2C8 is genetically polymorphic. Four variants, CYP2C8*2, CYP2C8*3, CYP2C8*4 and CYP2C8*5, which contain mutations in the coding regions have been reported to exhibit different enzyme activity as compared with CYP2C8*1.

Objective:  To determine the allele frequency of three codon-changing variants (CYP2C8*2, CYP2C8*3 and CYP2C8*4) in the Malaysian population.

Method:  Healthy unrelated volunteers from three major races in Malaysia were recruited. The study was approved by the local Research Ethics Committee. DNA was extracted using a standard protocol. A two-step multiplex PCR method was developed to detect three alleles of CYP2C8. PCR results were confirmed by subsequent direct DNA sequencing.

Result:  Only the Indians showed CYP2C8 polymorphism with allele frequency of 98% for CYP2C8*1, 0·8% for CYP2C8*2 and 1·2% for CYP2C8*3. CYP2C8*4 was not detected in any of the ethnic groups.

Conclusion:  To the best of our knowledge, the current study described, for the first time polymorphisms of CYP2C8 in Malaysian Indians.

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