Multigenerational analysis of spatial structure in the terrestrial, food-deceptive orchid Orchis mascula
Article first published online: 19 DEC 2008
© 2008 The Authors. Journal compilation © 2008 British Ecological Society
Journal of Ecology
Volume 97, Issue 2, pages 206–216, March 2009
How to Cite
Jacquemyn, H., Wiegand, T., Vandepitte, K., Brys, R., Roldán-Ruiz, I. and Honnay, O. (2009), Multigenerational analysis of spatial structure in the terrestrial, food-deceptive orchid Orchis mascula. Journal of Ecology, 97: 206–216. doi: 10.1111/j.1365-2745.2008.01464.x
- Issue published online: 11 FEB 2009
- Article first published online: 19 DEC 2008
- Received 3 April 2008; accepted 10 November 2008; Handling Editor: Honor Prentice
- micro-environmental variation;
- parentage analysis;
- seed flow;
- spatial genetic structure;
- spatial point pattern analysis
- 1In long-lived, terrestrial orchids, strong aggregation of adults and recruits within populations and pronounced spatial association between recruits and adults can be expected when seed dispersal is limited, probabilities of seed germination decrease with increasing distance from mother plants and/or not all mother plants contribute to future generations. When individuals are distributed evenly across life-history stages, these processes can also be expected to result in a significant fine-scale spatial genetic structure in recruits that will persist into the adult-stage class.
- 2We combined detailed spatial genetic and point pattern analyses across different generations with parentage analyses to elucidate the role of the diverse processes that might determine spatial structure in Orchis mascula.
- 3Analyses of spatial point patterns showed a significant association between adults and recruits and similar clustering patterns for both. Weak, but highly significant spatial genetic structure was observed in adults and recruits, but no significant differences were observed across life stages, indicating that the spatial genetic structure present in recruits persists into the adult stage.
- 4Parentage analyses highlighted relatively short seed dispersal distances (median offspring-recruitment distance: 1.55 and 1.70 m) and differential contribution of mother plants to future generations.
- 5Persistence of fine-scale spatial genetic structure from seedlings into the adult stage class is consistent with the life history of O. mascula, whereas relatively large dispersal distances of both pollen and seeds compared to the fine-scale clustering of adults and seedlings suggest overlapping seed shadows and mixing of genotypes within populations as the major factors explaining the observed weak spatial genetic structure.
- 6Nonetheless, comparison of the spatial association between recruits and adults with the genetic analysis of offspring-parent distances suggests that the tight clustering of recruits around adults was probably caused by decreasing probabilities of seed germination with increasing distance from mother plants.
- 7Synthesis. This study shows that the approach presented here, which combines spatial genetic and spatial pattern analyses with parentage analyses, may be broadly applied to other plant species to elucidate the processes that determine spatial structure within their populations.