Variation in gene expression of Andropogon gerardii in response to altered environmental conditions associated with climate change
Article first published online: 24 DEC 2009
© 2009 The Authors. Journal compilation © 2009 British Ecological Society
Journal of Ecology
Volume 98, Issue 2, pages 374–383, March 2010
How to Cite
Travers, S. E., Tang, Z., Caragea, D., Garrett, K. A., Hulbert, S. H., Leach, J. E., Bai, J., Saleh, A., Knapp, A. K., Fay, P. A., Nippert, J., Schnable, P. S. and Smith, M. D. (2010), Variation in gene expression of Andropogon gerardii in response to altered environmental conditions associated with climate change. Journal of Ecology, 98: 374–383. doi: 10.1111/j.1365-2745.2009.01618.x
- Issue published online: 25 JAN 2010
- Article first published online: 24 DEC 2009
- Received 16 June 2009; accepted 16 November 2009 Handling Editor: Joop Ouborg
- ecological genomics;
- enrichment analysis;
- gene function;
- Gene Ontology;
- precipitation variability;
- Rainfall Manipulation Plots;
- tallgrass prairie;
- transcription profile;
1. If we are to understand the mechanisms underlying species responses to climate change in natural systems, studies are needed that focus on responses of non-model species under field conditions. We measured transcriptional profiles of individuals of Andropogon gerardii, a C4 grass native to North American grasslands, in a field experiment in which both temperature and precipitation were manipulated to simulate key aspects of forecasted climate change.
2. By using microarrays developed for a closely related model species, Zea mays, we were able to compare the relative influence of warming versus altered soil moisture availability on expression levels of over 7000 genes, identify responsive functional groups of genes and correlate changes in gene transcription with physiological responses.
3. We observed more statistically significant shifts in transcription levels of genes in response to thermal stress than in response to water stress. We also identified candidate genes that demonstrated transcription levels closely associated with physiological variables, in particular chlorophyll fluorescence.
4.Synthesis. These results suggest that an ecologically important species responds differently to different environmental aspects of forecast climate change. These translational changes have the potential to influence phenotypic characters and ultimately adaptive responses.