SEARCH

SEARCH BY CITATION

Keywords:

  • agricultural pollution;
  • broom (Cytisus scoparius);
  • gorse (Ulex europaeus);
  • invasion ecology;
  • invasive plants;
  • N cycling;
  • N excess;
  • N isotopes;
  • riparian N cycling

Summary

  1. Top of page
  2. Summary
  3. Introduction
  4. Materials and methods
  5. Results
  6. Discussion
  7. Acknowledgements
  8. References

1. This study examined the seemingly paradoxical proliferation of invasive, N2-fixing broom (Cytisus scoparius) and gorse (Ulex europaeus) in N-saturated riparian areas of intensive agricultural land in Canterbury, New Zealand.

2. A field study of natural abundance δ15N suggested that broom and gorse along the Selwyn River fix approximately three times more N than they take up from soils, and are thus a potentially large source of N in the landscape. Broom N fixation rates based on mass balance calculations from a glasshouse study were similar.

3. In the controlled glasshouse study, broom grown at both c. 1× and 6.5× field NO3 supply fixed N at the same rate per unit biomass (0.061 mg N day−1 g−1 dry wt) over a 9-month period. Broom plants grown under the high-N supply, however, grew c. 1.6 times larger, and thus fixed more N per plant. Above-to-below-ground biomass ratios and %N in above- and below-ground pools were the same under the two levels of N supply.

4. Each broom plant in the greenhouse study contributed at least 0.02 g N year−1 to soils, but leaching from the soils was surprisingly low (<2% of total plant and soil stocks) suggesting that plants less than 1 year old are not contributing substantially to high NO3 concentrations in Selwyn ground and surface water.

5.Synthesis. This study shows that both broom and gorse growing in the Selwyn riparian area are an additional source of bioactive N in this N-saturated ecosystem. Additionally, broom grows more quickly as N availability increases and therefore fixes more N per plant. This suggests a positive feedback whereby agricultural nutrient pollution leads to increased per-plant N2 fixation in broom, and probably in gorse, given the taxonomic and physiological similarity of the species. The Selwyn is representative of a large number of New Zealand rivers with riparian zones that are dominated by invasive N2 fixers. The likelihood that these invasive plants increase the amount of bioactive N in rivers and downstream ecosystems presents new considerations and challenges for management.


Introduction

  1. Top of page
  2. Summary
  3. Introduction
  4. Materials and methods
  5. Results
  6. Discussion
  7. Acknowledgements
  8. References

In the last two decades New Zealand has experienced dramatic increases in land-use intensity, the area of farmland under irrigation and a c. 10-fold increase in the import and use of synthetic fertilizers (Parfitt et al. 2006). These changes contribute to growing concerns about nutrient pollution in freshwaters and nearshore areas. Exotic, fast-growing N2-fixing species, particularly broom (Cytisus scoparius) and gorse (Ulex europaeus), have proliferated in riparian areas and have largely replaced native plants, most of which were not N2 fixers. These invasive plants are thus potentially a major new source of bioactive N in rivers and downstream ecosystems.

This study examines the role of broom and gorse in the N dynamics of a degraded, nutrient-enriched river. The Selwyn (Canterbury Region, Fig. 1) is an alluvial river that flows through an intensively agricultural landscape before entering Te Waihora/Lake Ellesmere, a hypertrophic coastal lagoon. On average, c. 100 kg fertilizer N ha−1 year−1 and c. 150 kg fixed N ha−1 year−1 from cultivated legumes are added to soils in Canterbury (2001 values, Parfitt et al. 2006). Atmospheric deposition of reactive N is low (1–3 kg N ha year−1) in New Zealand’s South Island (Galloway et al. 2004) and has not increased substantially since the late 1800s. The Selwyn catchment is, ostensibly, N-saturated. For example, intensive water quality monitoring in 2004 showed that NO3-N concentrations increased by 10–100 fold in the c. 50 km between the foothills and the mouth of the Selwyn throughout the year. Such elevated concentrations of NO3 in ground and surface waters are a primary symptom of N excess at the catchment scale (Aber et al. 1998; Fenn et al. 1998). Increasing groundwater withdrawal for irrigation has led to reductions in the Selwyn summer discharge (McKerchar & Schmidt 2007) and may also contribute to increasing concentrations of NO3 in surface and ground waters. Although degraded, rivers such as a Selwyn still provide ecological services such as transport, transformation and sequestration of nutrients. For example, when the dissolved inorganic N load was experimentally increased by a factor of >30 over natural concentrations in a New England tidal river, the ecosystem continued to process c. 54% of the inorganic N load through biological uptake and denitrification (Drake et al. 2009).

image

Figure 1.  Map of the Selwyn River. Three major reaches, the upstream perennial (losing) reach, the intermittent reach, and the downstream perennial (gaining) reach are demarcated by dashed lines. Plant or water samples were collected at the numbered Rkm locations (distance in km from the river source).

Download figure to PowerPoint

The riparian vegetation of many degraded rivers in New Zealand is dominated by broom and gorse – closely related legumes of the subfamily Papilionoideae. These species were introduced to New Zealand for use as hedgerows in the early 1800s, but quickly escaped cultivation and became nuisance species. The ability of broom and gorse to fix N2 may contribute to their success in recently disturbed alluvial riparian areas which, under natural conditions, are relatively low-N environments (e.g. Rhoades et al. 2008). N2-fixing plants, however, are rare in the native riparian flora of New Zealand (Williams & Wiser 2004), suggesting that other life-history traits contribute to the proliferation of broom and gorse. For example, both are aggressive pioneer species that set large numbers of seeds, are adapted to frequent disturbance, drought, and inorganic soils (Pojar & MacKinnon 1994), and both have photosynthetic stems that improve water-use efficiency (Nilsen & Sharif 1983).

N2 fixers should have an advantage in low-N environments, but the proliferation of N2-fixing vegetation in the N-saturated landscape of Canterbury seems paradoxical. If NO3 is abundant in soils, uptake should be more efficient than N2 fixation which requires expenditure of c. 35% more C than root uptake (Pate & Layzell 1990). Given the physiological expense of N2 fixation and the high availability of NO3 in the Selwyn riparian area, one would expect plants in this habitat to derive N from soils. If the success of broom and gorse is based on their ability to fix N2, these plants should lose their competitive advantage and be replaced by non-N2 fixers as the availability of NO3 increases.

N2 fixers are the world’s largest natural source of bioactive N and they play a critical role by regulating N availability in early successional stages of ecosystem development (Chapin, Matson & Mooney 2002). A review of regulation of N2 fixation cited evidence that it is influenced by N availability (Hartwig 1998), while other studies have shown no effect of N supply on N2 fixation rate (e.g. Binkley, Senock & Cromack 2003). Few clear patterns are evident, but, in general, NO3 tends to inhibit fixation more than NH4+, and NO3 is very abundant in the Selwyn system.

The continued competitiveness of broom and gorse under high-N conditions may be at least partially explained if they were facultative N2 fixers, fixing less N as soil N availability increases. It is unknown, however, whether broom and gorse are obligate or facultative N2 fixers. Many N2 fixers appear to use an obligate strategy at natural N concentrations, especially in temperate zones (e.g. Menge & Hedin 2009), but frequently disturbed, high-light conditions such as those in the Selwyn should favour a facultative strategy according to modelled cost–benefit analysis (Menge, Levin & Hedin 2009).

Ultimately, the contribution of N2-fixing riparian species to N dynamics in the nutrient-polluted streams of Canterbury is unknown. This study was designed to describe and quantify N uptake and contribution by broom and gorse. I used two approaches – a field study examining N isotope composition of broom, gorse, surface water and ground water, and a controlled glasshouse mass balance study in which broom seedlings were grown under high and low-N supply – to answer three questions:

  • 1
     Do broom and gorse function as a source or a sink of N in the Selwyn River system?
  • 2
     If broom and gorse are fixing N2, do fixation rates decrease as NO3 concentrations in ground and surface water increase downstream, and do growth rates, above-ground : below-ground biomass ratios, or %N in plants change with NO3 availability?
  • 3
     If broom and gorse are fixing N2, what is their annual contribution of N to riparian soils and leaching?

Materials and methods

  1. Top of page
  2. Summary
  3. Introduction
  4. Materials and methods
  5. Results
  6. Discussion
  7. Acknowledgements
  8. References

Study Site

The Selwyn River originates in the foothills of New Zealand’s Southern Alps, and flows east for 58 km through the alluvial Central Plains of Canterbury (Fig. 1). It drains a catchment of 97 354 ha and is the largest tributary of Lake Ellesmere/Te Waihora, a hypertrophic coastal lagoon. In recent years the middle reaches of the Selwyn have dried completely during summer resulting in three distinct reaches: a 10-km perennial-flow reach from the headwater to where the river flows onto the plains, a 43-km intermittent reach which is frequently dry, and a 15-km perennial-flow groundwater-fed gaining reach near the river mouth (Larned et al. 2008; Fig. 1). Monthly monitoring during 2004 showed a consistent >10-fold increase in stream water NO3 concentrations between the headwaters (always <0.3 mg L−1) and the mouth (always >5.0 mg L−1).

The Selwyn’s riparian flora is dominated by exotic plants: broom, gorse, willow (Salix spp.), lupin (Lupinus spp.) and non-native grasses comprise >95% of vegetation cover (A. Hendry & D. C. Drake, unpubl. data). Only a few scattered native species such as willow weeds (Epilobium sp.) and mat-forming cushion plants (Raoulia sp.) persist. The average density of broom and gorse in the riparian area was 5.5 plants m−2, and the mean age was 4.5 years with no plants older than 7 years (A. Hendry and D.C. Drake, unpubl. data). For the purposes of this study, the riparian area is defined as all of the area surrounding the channel that is not actively farmed – this generally extends 50–500 m from the active channel, and is maintained in a disturbed state by channel migration.

Field Methods

Broom and gorse leaves were collected from seven sites [river kilometre (Rkm) 3, 7.4, 13.3, 38, 49, 55.4 and 59.5, Fig. 1] in September 2007 (spring). It is in spring when the highest concentration of N tends to be found in the leaves of most deciduous trees (e.g. Chapin & Kedrowski 1983) and therefore collection at this time should minimize the effect of seasonal N isotope fractionation resulting from N resorption. New growth foliage was collected from at least five different plants at each of two locations [near (<5 m) and far (>40 m) from the active channel] at each of the seven sites. Leaves were dried for at least 48 h at c. 100 °C and then ground to a fine powder. Equal weights of ground leaf material from the 5+ plants at each location were composited for δ15N analysis.

Surface water was collected for δ15NO3 and NO3-N determination in the late winter (September) 2007 at Rkm 3, 13.3 and 59.5, and mid-summer (December) 2008 at Rkm 3, 13.3, 49 and 59.5 (Fig. 1). Groundwater from a depth of c. 6 m was collected for δ15NO3 and NO3-N determination from one well at Rkm 12 in November 2007 and three wells at Rkm 12, 40 and 58 in December 2008.

Water was filtered through combusted GF/F filters and frozen in acid-washed bottles until analysis. Surface and ground water δ15NO3 was determined using protocols developed for the Lotic Inter-site Nitrogen eXperiment (LINX; http://www.biol.vt.edu/faculty/webster/linx/). Briefly, NO3 was isolated from water samples by driving off NH4+ by boiling water samples in an open container with MgO (conversion to gaseous NH3). Boiling reduced the sample volume to c. 100 mL. Devardas alloy, salt, additional MgO and acidified GF/F filter material sealed in waterproof but gas-permeable Teflon tape were added to the samples, and bottles were quickly sealed. The sealed samples were incubated for c. 48 h at 70 °C, and then for 14 days on a shaker table for c. 30 °C. Devardas alloy reduces NO3 to NH4+, which then is converted to gaseous NH3 and is adsorbed to the acidified filter material in the head space of sample containers. After incubation, the filter material was removed from Teflon pouches and stored in a desiccator for at least 5 days prior to shipping for analyses. All isotope samples were shipped to the University of California, Davis, Stable Isotope Facility for determination of δ15N and δ13C.

Glasshouse Methods

Study design

Only broom was used in the glasshouse study. All N inputs, standing stocks and outputs except gaseous losses were quantified for 40 different plants and their respective pots of soil between 2 June 2008 and 17 March 2009. This included: above- and below-ground broom biomass and N pools, extractable soil N, N fertilizer added to each pot, water volume and the cumulative mass of glasshouse tap water N (determined from concentrations of NH4+ and NO3) added to each pot, and NH4+, NO3 and total N (TN) in leachate from each pot. Because soils contained almost no organic matter (only a trace was detected), total soil N was not measured.

On 1 June 2008 eighty-five 10-L plastic pots, 25 cm in diameter, were filled with newly deposited river gravel and sand (henceforth ‘soils’) collected from Rkm 38–39 of the Selwyn. These were transported to a glasshouse at the University of Canterbury, Christchurch. The following day, c. 90 broom seedlings (above-ground height <30 cm, less than 2 months old) with their entire root systems were carefully excavated from within c. 500 m of the soil collection area. Sixty seedlings were planted, one per pot, and a random selection of 20 seedlings were kept for initial biomass and δ15N determination (described below). Fifteen pots were not planted and were used as controls for determination of soil N accumulation and leaching. Seedlings were allowed to acclimatize for 1 week before any fertilizer was added, and several seedlings that appeared to be unhealthy were replaced. An automatic drip irrigation system was used to water plants and the control pots individually. The volume of water added over each c. 2-week period was estimated from two hoses that drained into plastic jugs.

The low-N (tap water sourced from local groundwater) and high-N (tap water + fertilizer) treatments were loosely based on N concentrations observed in the Selwyn River, although it was not possible to measure the bulk flow of groundwater to quantify root exposure to NO3 in the field. Each week 5.0 mg N as KNO3 and 1.0 mg P as K2HPO4 was added to ‘high-N’ plants, and 1.0 mg P only was added to the ‘low-N’ plants. Fertilizer δ15N was +2.98‰. Low-N plants received only N dissolved in tap water, and tap water δ15N was not determined.

Leachate from all plants was collected in DI-rinsed plastic trays under each pot. Throughout most of the 9-month experiment the leachate trays contained only a small amount of water or were dry, but on two dates early in the experiment several trays filled with water, which was collected and frozen for later analysis. Glasshouse tap water was collected approximately once per month for N concentration analyses.

Broom plants were destructively harvested 2, 4, 7 and 9 months after planting. On each date five high-N plants, five low-N plants and three unplanted controls were harvested. Soils were sieved to retrieve all root material. Leachates containing N that seeped out of pots were collected by scrubbing trays out with DI water. The wash water volume was measured and a subsample was used for total N, NO3 and NH4+ determination. Soils in each pot were homogenized and subsamples of 100 g (particles <10 mm diameter) were collected and refrigerated until analysis.

Laboratory Methods

Above- and below-ground portions of each plant were washed thoroughly with tap water, dried at 100 °C and weighed. From each pot, 20-g soil subsamples were extracted with 100 mL 1M KCl. Samples in KCl were shaken vigorously and allowed to stand overnight. The following day the samples were re-shaken and allowed to settle, then filtered through pre-leached, 10-cm diameter GF/F Whatman filters and frozen for nutrient analyses. Soil subsamples were weighed for loss-on-ignition and bulk density determination.

For the first three harvests, entire above- and below-ground fractions of each plant were ground for δ15N determination so that results would be representative of the entire above- or below-ground pool. At the fourth harvest above-ground pools had become very large, so each plant was divided roughly into quarters radially, and one quarter was ground for isotope analysis.

Quantitative analyses

N isotope model

The two major N sources available to riparian broom and gorse in the Selwyn system are (i) fixed N2 and (ii) dissolved N in soil water. The δ15N values of the two sources were used as endmembers to estimate source contribution to plant N using a double endmember mixing model (eqn 1). NO3 comprised 97.0–99.8% of dissolved inorganic nitrogen in the Selwyn system during monthly sampling in 2004 (S. T. Larned, unpubl. data). The high permeability of Selwyn gravels suggests that shallow ground water (within 6 m of the surface) and surface water are intimately connected – i.e. dissolved NO3 in surface water is representative of the N that riparian plants access through uptake. The isotopic endmember value for N2 fixation was taken from a controlled glasshouse study which determined that broom grown in N-free soils (forcing 100% N fixation) had leaf δ15N of −0.64‰ (Watt et al. 2003). Equation 1 was used to calculate %N fixed from leaf δ15N (δ15Nleaf), mean δ15NO3 in ground or surface water collected from the site nearest to plant collection, and the 100% broom fixation value (δ15Nfix).

  • image(eqn 1)

The mass of N fixed by each plant upon destructive harvest in the glasshouse experiment was calculated using mass balance (eqn 2). Initial N pools and NO3-N in water and fertilizer (Nfert) were subtracted from the sum of all N pools at the end of the experiment:

  • image(eqn 2)

Paired t-tests were used to determine whether field δ15N values differed between species or with distance to the river (<5 m vs. >40 m from the active channel) and to compare plant size and N stocks over the course of the glasshouse experiment. A single factor anova and Tukey’s multiple comparison of δ15N values pooled by site (each site = 4) was used to determine whether plant δ15N differed along the length of the river and plant characteristics varied over time in the glasshouse experiment.

Results

  1. Top of page
  2. Summary
  3. Introduction
  4. Materials and methods
  5. Results
  6. Discussion
  7. Acknowledgements
  8. References

Field Study

N2 fixation estimates based on leaf δ15N of plants growing in the field suggest that broom and gorse in the Selwyn riparian area fix 66–88% of their foliar N (Table 1). All broom and gorse δ15N values from the field study were between the dissolved NO3 and 100% N fixation endmembers (eqn 1), lending further support to the assumption that these were the main sources of N in the Selwyn riparian area. The δ15N of both species was significantly lower (among sites anova< 0.01) in the middle reaches (Rkm 13.3, 37.9 and 48.9, Fig. 1, Table 1) relative to upper and lower reaches (Rkm 3, 7.4, 55.4 and 59.5, Tukey’s multiple comparisons), and both broom and gorse δ15N values were statistically equal within species between the upper and lower reaches.

Table 1.   δ15N and resulting estimates of %N (±SE) fixed in broom and gorse foliage along the length of the Selwyn River. Values for broom and gorse are averages of two samples (near and far from the channel), each composited from five plants – i.e. averages are representative of 10 plants. % N fixed was calculated based on N fixation and dissolved NO3 endmembers (eqn 1). Groups a and b were determined using an anova and Tukey’s multiple comparison of broom and gorse δ15N values pooled by site (each site = 4)
River km from headwatersAverage δ15N (‰)Foliar N fixed (%)
BroomGorseBroomGorse
3a1.0 ± 0.091.3 ± 0.1774.9 ± 6.769.5 ± 11.8
7.4a1.3 ± 0.040.8 ± 0.0468.2 ± 2.776.5 ± 3.05
13.3b0.8 ± 0.370.6 ± 0.0976.8 ± 28.480.5 ± 7.2
37.9b0.3 ± 0.050.1 ± 0.0386.3 ± 4.388.4 ± 2.7
48.9b1.0 ± 0.270.4 ± 0.0274.7 ± 20.184.5 ± 1.7
55a1.8 ± 0.511.3 ± 0.0666.7 ± 34.073.4 ± 4.4
59.5a1.7 ± 0.041.4 ± 0.2567.7 ± 2.772.4 ± 18.1

Average gorse δ15N was 0.83‰– slightly but significantly lower than the average broom δ15N of 1.12‰ (two-tailed, paired t-test = 0.04). There was no statistical difference in broom or gorse δ15N with distance from the river (within 5 m and >40 m from the active channel), and there was no pattern in broom or gorse δ13C measured in a subset of 15 samples – all values were within 0.5‰ of −25.5 ‰. Average foliar %N was 3.69 ± 0.52 for broom and 2.46 ± 0.48 for gorse.

In September (late winter) 2007, Selwyn River water δ15NO3 values ranged from 5.53‰ to 6.63‰, and groundwater δ15NO3 was within this range at 6.03‰. The standard deviation of five dissolved NO3 standards was 0.77‰. Surface water δ15NO3 in December 2008 (mid-summer) was also similar along most of the length of the river (3.05–3.30‰), and groundwater δ15NO3 at Rkm 40 and 58 was similar to surface water (2.13‰ and 3.09‰, respectively), although at Rkm 13.3 groundwater δ15NO3 was 8.25‰. On both dates surface water NO3-N concentrations increased from 0.3 to 0.5 mg L−1 in the headwaters to 6.2–7.1 mg L−1 in the lowest reaches. Groundwater NO3-N concentration also increased downstream from 0.9 mg L−1 at Rkm 12–6.3 mg L−1 at Rkm 58.

Glasshouse Study

N pools and conditions at the onset of the experiment

Soils collected from the Selwyn River riparian area contained <1% organic matter by weight. Nutrient concentrations were also very low; extractable (plant available) NH4+ was <0.001 mg N g−1 dry soil, extractable NO3 was generally 0.001–0.003 mg N g−1 dry soil, and extractable PO43+was usually below the detection limit of c. 0.0001 mg P g−1 dry soil. The mean, initial soil N standing stock in each pot was 6.3 ± 2.4 mg (Table 2).

Table 2.   Nitrogen pools, additions and a mass balance calculation of N fixation under high and low-N supply after 7 and 9 months of growth in the glasshouse. Plant and soil values are mean values of five replicates ± standard errors. Water N concentrations were measured in single samples over time and have no error estimates. Leachate samples were pooled for analysis and have no error estimates
  7 months (January 2009)9 months (March 2009)
 High NLow NHigh NLow N
TreatmentN (mg)N (mg)N (mg)N (mg)
N supplied to each pot + plantSeedlings10.8 ± 1.110.8 ± 1.110.8 ± 1.110.8 ± 1.1
Soil6.3 ± 2.46.3 ± 2.46.3 ± 2.46.3 ± 2.4
Water28.128.132.732.7
Fertilizer14501900
N pools at harvestPlants717.4 ± 75.6287.4 ± 46.5863.9 ± 59.9438.8 ± 12.5
Soil30.2 ± 4.522.7 ± 6.368.5 ± 9.153.0 ± 12.3
Leached N5.45.513.95.7
N fixed per plant (pools at harvest – supplied; mg) 562.8 ± 12.3270.3 ± 69.2706.6 ± 95.5447.8 ± 50.5

The mean dry weight of whole broom seedlings collected in July 2008 was 0.52 ± 0.08 g, and the average N content was 0.0108 ± 0.0012 g (c. 2% of dry wt). Above-ground : below-ground seedling biomass was c. 1:1.

N added over the experiment

Glasshouse tap water contained almost no NH4-N throughout the experiment (<0.001 mg L−1) while NO3-N increased over the summer and autumn from 0.6 to 3.2 mg L−1. Monthly average NO3-N concentrations multiplied by the volume of water added to each pot comprised the low-N treatment: 4.0 mg NO3-N had been added from tap water by the first destructive harvest, 11.9 mg by the second, 28.1 mg by the third, and 32.7 mg by the fourth (Table 2). High-N treatments received tap water N and were also fertilized with 5 mg NO3-N per week.

Broom growth and N mass balances

By the end of the 9-month glasshouse study, broom under high-N supply had grown 1.6 times larger than broom under low-N supply (Table 2, Fig. 2a). Above-ground : below-ground biomass increased from an initial value of c.1 to an average of c. 1.8 by the first destructive sampling (3 months), and remained at that level in both treatments until the end of the experiment (Fig. 2b). No consistent pattern in root or shoot δ15N was attributable to N supply (Fig. 3). %N was similar between treatments with the exception of the January (mid-summer) 2009 harvest in which the average N content of below-ground tissues in low-N plants was significantly lower than in high-N plants (2.17 ± 0.24% vs. 1.66 ± 0.24% respectively). Over the experiment, the average N content of stems was 2.37 (± 0.26) % and below-ground tissues was 1.92 (± 0.23) %.

image

Figure 2.  Average (±SE) biomass (a) and above-ground:below-ground biomass ratios (b) of broom grown under high (inline image) and low (inline image) N supply. *Significant differences (t-test < 0.05) between N treatments and differences over time (anova< 0.05) in biomass ratios are denoted by a,b.

Download figure to PowerPoint

image

Figure 3.  δ15N of above-and below-ground pools (±SE) in low-N (inline image) and high-N treatments (inline image).*Significant differences (t-test < 0.05) between treatments.

Download figure to PowerPoint

N fixation per gram of plant dry weight (gdw) derived from mass balances was remarkably similar in the high- and low-N treatments at 0.092 (±0.017) and 0.091 (±0.021) mg gdw−1 day−1 after 7 months and 0.061 (±0.007) and 0.062 (±0.006) mg gdw−1 day−1 at 9 months (Table 3). Broom δ15N in the high- and low-N treatments (Fig. 3) were also similar throughout the experiment providing additional evidence that N2 fixation rates per unit biomass were similar irrespective of N supply. I note that gaseous losses were not measured, and would result in higher estimated rates of N fixation. Average broom δ15N in the glasshouse experiment (excluding initial values) was −1.0 (±0.7) ‰, notably lower than the 100% fixation value from Watt et al. (2003) of −0.64‰. Fertilizer δ15N was +2.98‰ and cannot be responsible for the low broom δ15N, suggesting that the N fixation endmember here may be lower than the value determined by Watt et al. (2003).

Table 3.   Mean dry weights, N fixation rates and % of N fixed in broom (±SE) after 7 and 9 months of growth under controlled glasshouse conditions
 7 months (January 2009)9 months (March 2009)
High NLow NHigh NLow N
Mean dry weight whole plant (g)28.19 ± 1.9113.67 ± 4.6041.4 ± 3.6925.8 ± 1.35
N fixed (mg day−1)2.58 ± 0.391.24 ± 0.242.52 ± 0.261.60 ± 0.14
N fixed per g plant dry weight (mg day−1)0.092 ± 0.0170.091 ± 0.0210.061 ± 0.0070.062 ± 0.006
% N fixed estimated using mass balance74.7%85.7%74.7%90.0%

Soils subjected to all treatments (including unplanted controls) accumulated N, but soils in the high-N treatment accumulated significantly more N than those in the low-N treatment (Table 2, t-test < 0.05). At the end of the experiment soils in high-N treatment contained, on average, c. 4.7 ± 0.62 g N m−3 (c. 10.9×) more extractable N than they did initially. Soils in the low-N treatment contained 3.5 ± 0.80 g N m−3 (c. 8.4×) more, and unplanted control soils contained 2.5 ± 0.33 g N m−3 (c. 6.1×) more than they did initially. Each young plant, therefore, contributed c.1.3–2.9 g N m−3 year−1 to riparian soils, and plants contributed more N to soils as N load increased.

Soil leachates from pots with plants contained <2% of plant + soil N standing stocks. Thus plants and soils were retaining most of the N added to the pots and produced by N fixation. Leaching rates in the glasshouse experiment were c.1.45 g N m−2 year−1 in the control soils (which received only N in tap water), 0.76 g N m−2 year−1 in the low-N treatment soils, and 1.39 g N m−2 year−1 in the high-N treatment soils, suggesting that the presence of plants decreases N leaching rates. A statistical comparison is not possible, however, because samples were composited for analyses.

Discussion

  1. Top of page
  2. Summary
  3. Introduction
  4. Materials and methods
  5. Results
  6. Discussion
  7. Acknowledgements
  8. References

Only broom was used in the glasshouse study. It is closely related to gorse, it exhibited similar field δ15N values to gorse, and the two species grow interspersed and are equally abundant in the Selwyn system. I therefore apply findings of the glasshouse study to gorse, and in this discussion assume that both species are acting similarly in the Selwyn environment.

1. Do broom and gorse function as a source or a sink of N in the Selwyn River system?

N mass balances from the glasshouse study showed that broom fixed 75–90% of its total N. These rates are comparable to the field study in which natural abundance of 15N yielded estimates of 66–88% fixed N in broom and gorse foliage. Both results suggest that broom and gorse fix approximately three times more N than they take up, and, given their abundance, are therefore probably an important source of bioactive N in the ecosystem.

2. If broom and gorse are fixing N2, do fixation rates decrease as NO3 concentrations in ground and surface water increase downstream, and do growth rates, above-ground : below-ground biomass ratios, or %N change with NO3 availability?

In the glasshouse study, N supply had no effect on N2 fixation per unit biomass, suggesting an obligate strategy over very different levels of N supply (c. 1–6.5 × the already elevated field N supply), and contradicting the expectation of a facultative strategy. N supply did not affect above-ground : below-ground biomass or %N in tissues. Growth, however, was 1.6 times higher under the high-N supply, with the net effect of increasing per-plant N2 fixation by 1.6-fold.In the field study, leaf δ15N of both species was significantly lower in the middle (intermittent flow) reach relative to permanently flowing upper and lower reaches of the Selwyn, suggesting that plants in the middle reach fixed 10–11% more of their N than plants in the upper and lower reaches. Thus N fixation per unit biomass appears to be somewhat affected by a factor other than N supply in the field. Water availability is the most obvious difference between the reaches, but leaf δ13C showed no evidence of increased water stress in the middle reach or with distance from the channel. This finding may warrant additional study, although it should be noted that estimation of % N fixed in plant tissues using only δ15N is limited by several factors, including uncertainty about endmembers. In this study, for example, ground and surface water δ15NO3 at all sites was c. 3‰ lower in October (spring) 2007 than in December (summer) 2008, and plant NO3 uptake probably also changes considerably over the year. The N fractionation from soil source to plant leaf is affected by many factors and also potentially confounds leaf δ15N-based N fixation estimates. Where soil δ15N is near +6‰, as in the Selwyn system, the difference between leaf and soil δ15N is largely attributable to fractionation and can vary from 5‰ to +5‰ (Craine et al. 2009). Additionally, broom support arbuscular mycorrhizae, and they show a greater range and higher mean leaf δ15N than N2 fixers supporting ericoid or ectomycorrhizae (Craine et al. 2009).

3. If broom and gorse are fixing N2, what is their annual contribution of N to riparian soils and leaching?

The glasshouse experiment demonstrated a significant, but relatively small contribution of mineral (extractable) N to riparian soils. Broom plants increased N accumulation in soils over controls by c. 36% in the low-N treatment, and by 77% in the high-N treatment. Leaching of total N from pots containing broom (Table 2) was equal to or lower than N leaching from unplanted control pots. Leaching of N from the high-N treatment, however, was more than double that from the low-N treatment. Leaching rates measured here were within the range of values for N-saturated forest soils (e.g. Borken & Matzner 2004).Overall, contributions from young broom and gorse to N leaching and mineral N pools in newly deposited Selwyn soils were small compared to the c. 250 kg fertilizer and pasture legume N ha−1 year−1 added to soils in Canterbury (Parfitt et al. 2006). Young broom and gorse are probably not contributing substantially to the high concentrations of NO3 observed in surface and ground water. Instead, NO3 is efficiently intercepted by these plants and strongly conserved in both soils and plants during at least the first year of growth. Broom and gorse only live to ≤7 years in the Selwyn riparian, and most of the N stored in plant tissues is probably released when the plants die and decay – i.e. when areas supporting broom and gorse are reworked by floods. Selwyn riparian soils are C-poor (<0.1% organic matter by weight) suggesting that most senesced and dead broom and gorse decays or is exported very quickly and that little N is stored in riparian soils as recalcitrant organic matter (e.g. lignins).

A positive feedback between N supply and per-plant and per-unit area N2 fixation

Introduction of new N sources (such as agricultural pollution) to an ecosystem frequently reduces the competitive ability of legumes and inhibits symbiotic N2 fixation (Hartwig 1998; Menge, Levin & Hedin 2009). Under assumptions of self-regulation or facultative N2 fixation I expected to observe reduced rates of N fixation in plants grown under higher N supply, but this was contradicted by both field and glasshouse experiments. Instead, under the increased N supply, N fixation rates per unit biomass remained the same while the plants grew faster, resulting in increased per-plant N2 fixation. This, coupled with high rates of N2 fixation measured in the field, suggests that broom and gorse in the Selwyn riparian are N-limited even under the current conditions of high NO3-N availability. Under the range examined here, a 10% increase in NO3 supply resulted in an additional 34.5 mg of N2 fixed by each plant per year. Agricultural nutrient pollution is therefore almost certainly stimulating growth of broom and gorse and resulting in an increase in the amount of N2 fixed in the ecosystem per unit time.

Other factors that may limit the growth of broom and gorse in the Selwyn riparian include herbivory, limitation by nutrients other than N, and light and water availability. Herbivory on broom and gorse is very light as these species are unpalatable to livestock. There is also little visible evidence of insect damage (D.C. Drake, pers. obs.). Phosphorus is the most likely alternative limiting nutrient, but P fertilizers are used heavily in New Zealand pasture agriculture (e.g. they comprise c. 91% of total P concentrations in fresh water; Gillingham & Thorrold 2000), and P was added to broom in the glasshouse to simulate agricultural pollution. This and the fact that broom and gorse fix such a large fraction of the N in their tissues strongly suggest N-limitation in the Selwyn riparian. Light may limit growth in the field where plant densities are high and plants are older and larger. The density of broom in the glasshouse, however, was c. 15 plants m−2, or about three times the average density in the Selwyn riparian (A. Hendry & D. C. Drake unpubl. data) and plants showed no sign of light limitation. I conclude that light limitation is rare or unlikely during at least the first year of growth in the field. Broom and gorse are phreatophytes and are probably not severely limited by water availability. This is supported by broom and gorse δ13C – severe water stress would probably be indicated by higher δ13C in the drier, intermittent reaches, but this was not observed.

The N2 fixation strategy in the Selwyn River environment

High channel migration rates in many of New Zealand’s alluvial rivers maintain riparian areas in an early successional state, populated mainly by young, fast-growing, invasive legumes. Frequently disturbed environments should favour facultative over obligate N2 fixers as long as the costs of being facultative (regulation of physiology and mechanisms required to sense and respond to N availability) are relatively low (Menge, Levin & Hedin 2009). Broom, however, used an obligate strategy under a wide range of NO3-N supplied in the glasshouse study, and field results suggest obligate N fixation in both species. It is unknown whether a facultative N-fixing strategy might be triggered as N availability continues to increase or as the relative availability of P, light, CO2 or other potentially limiting resources changes.

This study raises a new concern about the interplay of invasive N-fixing species and nutrient pollution, and is of particular relevance given the recent, anthropogenic doubling (or more) of bioactive N availability at a global scale (Vitousek et al. 1997). The sequestration of nutrients by vegetation is used to manage nutrient pollution and is an important part of the ability of natural river ecosystems to maintain water quality. This study, however, shows that invasive broom and gorse in early successional riparian areas are likely to be a major, additional N source under nutrient-polluted conditions. These obligate N2 fixers can interact with N pollution in a positive feedback, with increased N availability from agricultural pollution or other sources resulting in higher plant growth rates, and a net increase in the amount of N2 fixed at the ecosystem scale.

Acknowledgements

  1. Top of page
  2. Summary
  3. Introduction
  4. Materials and methods
  5. Results
  6. Discussion
  7. Acknowledgements
  8. References

I sincerely thank Ann Hendry, Jo Bind, Clive Howard-Williams, Trevor Partridge, Dave Arscott, David Conder, John Carter, Greg Kelly, Rose Sephton-Poultney, the Editors at Journal of Ecology and several anonymous reviewers for their help and input through the process of designing, conducting, and writing up this study. This research was supported by NIWA Capability Funds CPAB075 and CPAB085.

References

  1. Top of page
  2. Summary
  3. Introduction
  4. Materials and methods
  5. Results
  6. Discussion
  7. Acknowledgements
  8. References
  • Aber, J., McDowell, W., Nadelhoffer, K., Magill, A., Berntson, G., Kamakea, M., McNulty, S., Currie, W., Rustad, L. & Fernandez, I. (1998) Nitrogen saturation in temperate forest ecosystems. BioScience, 48, 921934.
  • Binkley, D., Senock, R. & Cromack, K. (2003) Phosphorus limitation on nitrogen fixation by Falcataria seedlings. Forest Ecology and Management, 186, 171176.
  • Borken, W. & Matzner, E. (2004) Nitrate leaching in forest soils: an analysis of long-term monitoring sites in Germany. Journal of Plant Nutrition and Soil Science, 167, 277283.
  • Chapin III, F.S. & Kedrowski, R.A. (1983) Seasonal changes in nitrogen and phosphorus fractions and autumn retranslocation in evergreen and deciduous taiga trees. Ecology, 64, 376391.
  • Chapin III, F.S., Matson, P.A. & Mooney, H.A. (2002) Terrestrial nutrient cycling. Principles of Terrestrial Ecosystem Ecology (eds F.S.ChapinIII, P.A.Matson & H.A.Mooney), pp. 197223. Springer-Verlag, New York.
  • Craine, J.M., Elmore, A.J., Marcos, P.M., Aidar, M.B., Dawson, T.E., Hobbie, E.A. et al. (2009) Research review. Global patterns of foliar nitrogen isotopes and their relationships with climate, mycorrhizal fungi, foliar nutrient concentrations, and nitrogen availability. New Phytologist, 183, 980992.
  • Drake, D.C., Peterson, B.J., Galván, K.A., Deegan, L.A., Fleeger, J.W., Friedrichs, C.T., Hopkinson, C.S., Johnson, J.M., Koop-Jakobsen, K., Lemay, L.E. & Picard, C. (2009) Salt marsh ecosystem responses to nutrient enrichment: a paired 15N tracer study. Ecology, 90, 25352546.
  • Fenn, M.E., Poth, M.A., Aber, J.D., Baron, J.S., Bormann, B.T., Johnson, D.W., Lemly, A.D., McNulty, S.G., Ryan, D.F. & Stottlemyer, R. (1998) Nitrogen excess in North American ecosystems: predisposing factors, ecosystem responses, and management strategies. Ecological Applications, 8, 706733.
  • Galloway, J.N., Dentener, F.J., Capone, D.G., Boyer, E.W., Howarth, R.W., Seitzinger, S.P. et al. (2004) Nitrogen cycles: past, present, and future. Biogeochemistry, 70, 153226.
  • Gillingham, A.G. & Thorrold, B.S. (2000) A review of New Zealand research measuring phosphorus in runoff from pasture. Journal of Environmental Quality, 29, 8896.
  • Hartwig, U.A. (1998) The regulation of symbiotic N2 fixation: a conceptual model of N feedback from the ecosystem to the gene expression level. Perspectives in Plant Ecology, Evolution and Systematics, 1, 92120.
  • Larned, S.T., Hicks, D.M., Schmidt, J., Davey, A.J.H., Dey, K., Scarsbrook, M., Arscott, D.B. & Woods, R.A. (2008) The Selwyn River of New Zealand: a benchmark system for alluvial river plain rivers. River Research and Applications, 24, 121.
  • McKerchar, A.I. & Schmidt, J. (2007) Decreases in low flows of the lower Selwyn River? New Zealand Journal of Hydrology, 48, 6372.
  • Menge, D.N.L. & Hedin, L.O. (2009) Nitrogen fixation in different biogeochemical niches along a 120 000-year chronosequence in New Zealand. Ecology, 90, 21902201.
  • Menge, D.N.L., Levin, S.A. & Hedin, L.O. (2009) Facultative versus obligate nitrogen fixation strategies and their ecosystem consequences. American Naturalist, 17, 465477.
  • Nilsen, E.T. & Sharif, M.R. (1983) Carbon isotopic composition of legumes with photosynthetic stems from mediterranean and desert habitats. American Journal of Botany, 84, 17071713.
  • Parfitt, R.L., Schipper, L.A., Baisden, W.T. & Elliot, A.H. (2006) Nitrogen inputs and outputs for New Zealand in 2001 at national and regional scales. Biogeochemistry, 80, 7188.
  • Pate, J.S. & Layzell, D.B. (1990) Energetics and biological costs of nitrogen. The Biochemistry of Plants, a Comprehensive Treatise: Intermediary Nitrogen Metabolism Assimilation, Vol 16 (eds P.K.Stumpf, E.E.Conn, B.J.Miflin & P.J.Lea), pp. 142. Academic Press, San Diego.
  • Pojar, J. & MacKinnon, A. (eds) (1994) Plants of the Pacific Northwest Coast. Lone Pine, Vancouver.
  • Rhoades, C., Binkley, D., Oskarsson, H. & Stottlemyer, R. (2008) Soil nitrogen accretion along a floodplain terrace chronosequence in northwest Alaska: influence of the nitrogen-fixing shrub Shepherdia canadensis. Ecoscience, 15, 223230.
  • Vitousek, P.M., Mooney, H.A., Lubchenco, J. & Melillo, J.M. (1997) Human domination of Earth’s ecosystems. Science, 277, 494499.
  • Watt, M.S., Clinton, P.W., Whitehead, D., Richardson, B., Mason, E.G. & Leckie, A.C. (2003) Above-ground biomass accumulation and nitrogen fixation of broom (Cystisus scoparius) growing with juvenile Pinus radiata on a dryland site. Forest Ecology and Management, 184, 93104.
  • Williams, P.A. & Wiser, S. (2004) Determinants of regional and local patterns in the floras of braided riverbeds in New Zealand. Journal of Biogeography, 31, 13551372.