• British total diet study;
  • estimated average requirement;
  • folates;
  • high-performance liquid chromatography;
  • 5-methyltetrahydrofolate

5-methyltetrahydrofolate, the most bioavailable folate was measured by high-performance liquid chromatography in the British total diet study, a survey designed primarily to monitor food contaminants. A total 5 MeTHF of 130.1 μg (±21.7, n=6) was measured in the food groups which made up the average daily food intake. This value compares well to the estimated average requirement (EAR) of folate recently set by the Department of Health at 150 μg/day. The survey present here is an accurate reflection of folate consumed as all food samples were prepared ready for consumption.