Multiparameter phenotyping of T-cell subsets in distinct subgroups of patients with pulmonary sarcoidosis
Article first published online: 13 JUL 2011
© 2011 The Association for the Publication of the Journal of Internal Medicine
Journal of Internal Medicine
Volume 271, Issue 1, pages 90–103, January 2012
How to Cite
Wikén, M., Grunewald, J., Eklund, A. and Wahlström, J. (2012), Multiparameter phenotyping of T-cell subsets in distinct subgroups of patients with pulmonary sarcoidosis. Journal of Internal Medicine, 271: 90–103. doi: 10.1111/j.1365-2796.2011.02414.x
- Issue published online: 16 DEC 2011
- Article first published online: 13 JUL 2011
- Accepted manuscript online: 17 JUN 2011 07:20AM EST
- flow cytometry;
- lung disease;
- T cell
Abstract. Wikén M, Grunewald J, Eklund A, Wahlström J (Respiratory Medicine Unit, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden). Multiparameter phenotyping of T-cell subsets in distinct subgroups of patients with pulmonary sarcoidosis. J Intern Med 2012; 271: 90–103.
Objectives. Sarcoidosis is an inflammatory disorder in which elevated numbers of activated T cells are found in the lung. HLA-DRB1*0301pos (DR3pos) patients are characterized by good prognosis and an accumulation of lung CD4pos T cells expressing the T-cell receptor (TCR) gene segment AV2S3. Our aim was to phenotype lung and blood T-cell subsets in distinct patient groups to better understand the function of these subsets.
Design. Bronchoalveolar lavage (BAL) fluid and whole blood were obtained from a total of 22 patients with sarcoidosis, of whom 11 were DR3pos. Using eight-colour flow cytometry, phenotyping of T cells was performed with regard to CD3, CD4, CD8, CD25, CD27, CD45RO, CD57, CD69, CD103, FOXP3 and TCR AV2S3.
Results. DR3pos patients had fewer FOXP3pos (regulatory) CD45ROpos (memory) BAL T cells than DR3neg patients. Fewer AV2S3pos T cells were FOXP3pos, compared with AV2S3neg cells, thus indicating an effector function and not a regulatory role for this subset. Fewer lung and blood AV2S3pos T cells were CD25pos CD27pos, and more were CD25neg CD27neg and CD69pos, compared with AV2S3neg T cells, indicating a higher degree of differentiation and activation in both compartments.
Conclusion. Our main findings were a lower proportion of regulatory T cells in DR3pos patients, together with the accumulation of AV2S3pos T cells with a highly activated effector phenotype in the lungs of these patients. This may provide for efficient elimination of a harmful antigen in DR3pos patients and could thus help to explain the spontaneous recovery typically seen in these patients.