The etching of thick Araldite-embedded sections for scanning electron microscopy



A rapid method of correlation of information from the images of the scanning electron microscope, the transmission electron microscope and the light microscope, from biological tissues embedded in epoxy-resin. It allows a perception in depth at the SEM level of areas already defined by the other two modes. One micrometre sections of Araldite-embedded tissues are cut, dried onto glass cover slips, and the embedding medium etched away, with a saturated solution of NaOH in absolute ethanol. After washing in absolute ethanol and distilled water the resultant osmicated ‘skeleton’ is coated and viewed in the SEM.