Improvement in microscope photometry by voltage to frequency conversion: analogue measurement and digital processing
Article first published online: 2 AUG 2011
1981 Blackwell Science Ltd
Journal of Microscopy
Volume 124, Issue 2, pages 211–218, November 1981
How to Cite
Freitas, M. I., Giordano, P. A. and Bottiroli, G. (1981), Improvement in microscope photometry by voltage to frequency conversion: analogue measurement and digital processing. Journal of Microscopy, 124: 211–218. doi: 10.1111/j.1365-2818.1981.tb00315.x
- Issue published online: 2 AUG 2011
- Article first published online: 2 AUG 2011
- Revised paper accepted 15 July 1981
A system for digital measurement of microscope photometric signals, based on Voltage to Frequency Conversion (VFC) and suitable for automatic data processing, has been devised.
Advantages and disadvantages are illustrated with special emphasis on the comparison between photocurrent measuring and photon counting techniques. A statistical analysis of the results allows the calibration of VFC net counts versus detected photons. A biological application is reported; this shows the suitability of the system even to low light levels.