Simple optimization procedure for objective lens correction collar setting


Professor Tony Wilson. Fax: +44 (0)1865 273905; e-mail:


We present a new method for setting a coverglass correction collar on an objective lens. Axial scans across the interface between the specimen volume and the slide are used together with a quantitative function of merit to determine the optimum setting of the correction collar. The method, which simplifies the adjustment for the user and reduces photobleaching, was implemented within the software environment of a scanning microscope system.