A note on the stereological implications of irregular spacing of sections
Article first published online: 7 JUL 2006
Journal of Microscopy
Volume 222, Issue 3, pages 177–181, June 2006
How to Cite
BADDELEY, A., DORPH-PETERSEN, K.A. and VEDEL JENSEN, E.B. (2006), A note on the stereological implications of irregular spacing of sections. Journal of Microscopy, 222: 177–181. doi: 10.1111/j.1365-2818.2006.01585.x
- Issue published online: 7 JUL 2006
- Article first published online: 7 JUL 2006
- Received 28 April 2005; accepted 19 January 2006
- Cavalieri estimator;
- coefficient of error (CE);
- systematic sampling;
- variance formulae.
Stereological methods for serial sections traditionally assume that the sections are exactly equally spaced. In reality, the spacing and thickness of sections can be quite irregular. This may affect the validity and accuracy of stereological techniques, especially the Cavalieri estimator of volume. We present a new formula for the accuracy of the Cavalieri estimator that includes the effect of random variability in section spacing. A modest amount of variability in section spacing can cause a substantial increase in estimator variance.