We are deeply saddened that we must report the loss of our friend and colleague Dr. Nicolas Boisset who passed away on January 4, 2008.
Comparison of single-particle analysis and electron tomography approaches: an overview
Article first published online: 27 NOV 2008
© 2008 The Authors Journal compilation © 2008 The Royal Microscopical Society
Journal of Microscopy
Volume 232, Issue 3, pages 562–579, December 2008
How to Cite
JONIĆ, S., SORZANO, C.O.S. and BOISSET, N. (2008), Comparison of single-particle analysis and electron tomography approaches: an overview. Journal of Microscopy, 232: 562–579. doi: 10.1111/j.1365-2818.2008.02119.x
- Issue published online: 27 NOV 2008
- Article first published online: 27 NOV 2008
- Received 4 July 2007; accepted 2 March 2008
- Electron tomography;
- single-particle analysis;
- three-dimensional reconstruction;
- transmission electron microscopy
Three-dimensional structure of a wide range of biological specimens can be computed from images collected by transmission electron microscopy. This information integrated with structural data obtained with other techniques (e.g., X-ray crystallography) helps structural biologists to understand the function of macromolecular complexes and organelles within cells. In this paper, we compare two three-dimensional transmission electron microscopy techniques that are becoming more and more related (at the image acquisition level as well as the image processing one): electron tomography and single-particle analysis. The first one is currently used to elucidate the three-dimensional structure of cellular components or smaller entire cells, whereas the second one has been traditionally applied to structural studies of macromolecules and macromolecular complexes. Also, we discuss possibilities for their integration with other structural biology techniques for an integrative study of living matter from proteins to whole cells.