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Keywords:

  • Cameleon;
  • Ca2+ imaging;
  • FRET;
  • plant mitochondria

Summary

Mitochondria are key organelles involved in many aspects of plant physiology and, their ability to generate specific Ca2+ signatures in response to abiotic and biotic stimuli has been reported as one of their roles. The recent identification of the mammalian mitochondrial Ca2+ uniporter opens a new research area in plant biology. To study the mitochondrial Ca2+ handling, it is essential to have a reliable probe. Here we have reported the generation of an Arabidopsis transgenic line expressing the genetically encoded probe Cameleon D3cpv targeted to mitochondria, and compared its properties with the already known Cameleon YC3.6.