Defining the Gonadotrophin-Releasing Hormone Neuronal Network: Transgenic Approaches to Understanding Neurocircuitry

The gonadotrophin-releasing hormone (GnRH) neurones are the final downstream effector neurones driving the central regulation of reproductive function and fertility in all mammalian species. Although it is abundantly clear that successful fertility relies upon the communication of a variety signals regarding internal and external cues to the GnRH neuronal population, how this is achieved remains poorly understood. A range of technical limitations has posed significant hurdles to defining, with any certainty, the complexities of the synaptic neuronal network regulating GnRH neurones. However, recent advances in transgenic technology have opened up new avenues to reconsider questions aimed at understanding this critical network. This article addresses some of the latest advances that use transgenic mouse models as tools to understand the neuronal circuitry underpinning the regulation of the GnRH neurones. By incorporating standard morphological and viral tract tracing techniques with innovative transgenic tools, recent studies have uncovered previously unappreciated qualities of the GnRH neurone, including extensive dendritic lengths, numerous somal and dendritic spines and plasticity over pubertal development, along with beginning to define the primary and higher-order afferents that make up the GnRH neuronal network.