Preliminary results from the present study were communicated at the 11th EAVPT International Congress held on July 12–16, 2009 in Leipzig.
Effects of flunixin meglumine and ketoprofen on mediator production in ex vivo and in vitro models of inflammation in healthy dairy cows
Article first published online: 24 JUN 2012
© 2012 Blackwell Publishing Ltd
Journal of Veterinary Pharmacology and Therapeutics
Volume 36, Issue 2, pages 130–139, April 2013
How to Cite
DONALISIO, C., BARBERO, R., CUNIBERTI, B., VERCELLI, C., CASALONE, M. and RE, G. (2013), Effects of flunixin meglumine and ketoprofen on mediator production in ex vivo and in vitro models of inflammation in healthy dairy cows. Journal of Veterinary Pharmacology and Therapeutics, 36: 130–139. doi: 10.1111/j.1365-2885.2012.01396.x
- Issue published online: 8 MAR 2013
- Article first published online: 24 JUN 2012
- (Paper received 22 July 2011; accepted for publication 11 March 2012)
Donalisio, C., Barbero, R., Cuniberti, B., Vercelli, C., Casalone, M., Re, G. Effects of flunixin meglumine and ketoprofen on mediator production in ex vivo and in vitro models of inflammation in healthy dairy cows. J. vet. Pharmacol. Therap. 36, 130–139.
In this study, ex vivo assays were carried out in dairy cows to evaluate the anti-inflammatory effects of two nonsteroidal anti-inflammatory drugs: ketoprofen (KETO) and flunixin meglumine (FM). Twelve healthy Holstein dairy cattle were randomly allocated to two groups (n=6): group 1 received FM and group 2 received KETO at recommended therapeutic dosages. The anti-inflammatory effects of both drugs were determined by measuring the production of coagulation-induced thromboxane B2 (TXB2), lipopolysaccharides (LPS) (10 μg/mL)-induced prostaglandin E2 (PGE2), and calcium ionophore (60 μm)-induced leukotrien B4 (LTB4). Cytokine production was assessed by measuring tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ) and interleukin-8 (CXCL8) concentrations after incubation in the presence of 10 μg/mL LPS. The IC50 of FM and KETO was determined in vitro by determining the concentration of TXB2 and PGE2 in the presence of scalar drug concentrations (10−9–10−3 m). Both FM and KETO inhibited the two COX isoforms in vitro, but showed a preference for COX-1. FM and KETO showed similar anti-inflammatory effects in the cow.