Characterization of full-length hepatitis C virus genotype 4 sequences

Authors

  • J. Timm,

    1. Partners AIDS Research Center, Infectious Disease Division, Massachusetts General Hospital, Boston, MA, USA
    2. Department of Medicine, Ruhr-University Bochum, Bochum, Germany
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    • *

      J. Timm and M. Neukamm contributed equally to this work.

  • M. Neukamm,

    1. Partners AIDS Research Center, Infectious Disease Division, Massachusetts General Hospital, Boston, MA, USA
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    • *

      J. Timm and M. Neukamm contributed equally to this work.

  • T. Kuntzen,

    1. Partners AIDS Research Center, Infectious Disease Division, Massachusetts General Hospital, Boston, MA, USA
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  • A. Y. Kim,

    1. Partners AIDS Research Center, Infectious Disease Division, Massachusetts General Hospital, Boston, MA, USA
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  • R. T. Chung,

    1. Partners AIDS Research Center, Infectious Disease Division, Massachusetts General Hospital, Boston, MA, USA
    2. Gastrointestinal Unit, Massachusetts General Hospital, Boston, MA, USA
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  • C. Brander,

    1. Partners AIDS Research Center, Infectious Disease Division, Massachusetts General Hospital, Boston, MA, USA
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  • G. M. Lauer,

    1. Partners AIDS Research Center, Infectious Disease Division, Massachusetts General Hospital, Boston, MA, USA
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  • B. D. Walker,

    1. Partners AIDS Research Center, Infectious Disease Division, Massachusetts General Hospital, Boston, MA, USA
    2. Howard Hughes Medical Institute, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
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  • T. M. Allen

    1. Partners AIDS Research Center, Infectious Disease Division, Massachusetts General Hospital, Boston, MA, USA
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Dr Todd M. Allen, MGH-East, CNY 6616, 149, 13th Street, Charlestown, MA 02129, USA.
E-mail: tallen2@partners.org

Abstract

Summary.  Over 85% of the world's nearly 170 million hepatitis C virus (HCV)-infected subjects exist in regions of Africa, Southeast Asia and Middle Eastern countries where genotypes 4–6 are very common. In particular, HCV genotype 4 is highly prevalent in Egypt with more than 19% of the population infected and chronic HCV representing one of the top five leading causes of death, due in part to ineffective interferon alpha treatment against this genotype. Despite this, very little work has been carried out to characterize the sequence diversity of genotype 4, which will be critical to the development of effective vaccines and antiviral therapies against this genotype. As a result of the paucity of sequence data available for HCV genotype 4, for which only one full genome sequence is currently available, we were interested in characterizing additional genotype 4 sequences and to provide reagents for amplification of this genotype. Here we describe seven unique HCV genotype 4a full genomes, in addition to a single genotype 4d genome, and characterize their sequence diversity in relation to other more closely characterized HCV genotypes.

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