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Identifying Canadian mosquito species through DNA barcodes

Authors

  • A. CYWINSKA,

    Corresponding author
    1. 1 Department of Biological Sciences, Brock University, St. Catharines, Ontario, Canada and 2Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada
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  • 1 F. F. HUNTER,

    1. 1 Department of Biological Sciences, Brock University, St. Catharines, Ontario, Canada and 2Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada
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  • and 1 P. D. N. HEBERT 2

    1. 1 Department of Biological Sciences, Brock University, St. Catharines, Ontario, Canada and 2Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada
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Alina Cywinska, Department of Biological Sciences, Brock University, St. Catharines, Ontario L2S 3A1, Canada. Tel.: + 1 905 688 5550 (ext. 3879); Fax: + 1 905 688 1855; E-mail: alcywin@hotmail.com

Abstract

Abstract A short fragment of mt DNA from the cytochrome c oxidase 1 (CO1) region was used to provide the first CO1 barcodes for 37 species of Canadian mosquitoes (Diptera: Culicidae) from the provinces Ontario and New Brunswick. Sequence variation was analysed in a 617-bp fragment from the 5′ end of the CO1 region. Sequences of each mosquito species formed barcode clusters with tight cohesion that were usually clearly distinct from those of allied species. CO1 sequence divergences were, on average, nearly 20 times higher for congeneric species than for members of a species; divergences between congeneric species averaged 10.4% (range 0.2–17.2%), whereas those for conspecific individuals averaged 0.5% (range 0.0–3.9%).

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