‡Pioneer Hi-Bred Int., Department of Research Specialists, Johnston, 1A 501131, USA.
RFLP analysis of genetic variation in North American populations of the fall armyworm moth Spodoptera frugiperda (Lepidoptera: Noctuidae)
Article first published online: 28 JUN 2008
Volume 1, Issue 4, pages 199–208, December 1992
How to Cite
LU, Y.-J., ADANG, M. J., ISENHOUR, D. J. and KOCHERT, G. D. (1992), RFLP analysis of genetic variation in North American populations of the fall armyworm moth Spodoptera frugiperda (Lepidoptera: Noctuidae). Molecular Ecology, 1: 199–208. doi: 10.1111/j.1365-294X.1992.tb00178.x
- Issue published online: 28 JUN 2008
- Article first published online: 28 JUN 2008
- Received 12 February 1992; revision received 14 September 1992; accpeted 16 September 1992
- fall armyworm;
- genetic variation;
- molecular markers;
- Spodoptera frugiperda
A high level of genetic variability was detected in North American fall armyworm (FA W), Spodoptera frugiperda (J. E. Smith), populations by restriction fragment length polymorphism (RFLP) analysis of genomic DNA. In nearly all cases individual larvae could be differentiated using 22 probe-enzyme combinations. Laboratory colonies formed distinct groups based on RFLP analysis. Individuals from a colony of the previously determined ‘rice strain’ formed a group showing obvious deviation in RFLP patterns from the other five populations, which included a population from a ‘com strain’ colony. The results were consistent with previous studies using allozymes which indicated that there are two genetically differentiated forms of the fall armyworm. Diagnostic markers were also found that distinguish these two groups.